Gould T J, Bowenkamp K E, Larson G, Zahniser N R, Bickford P C
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262, USA.
Brain Res. 1995 Jul 3;684(2):150-8. doi: 10.1016/0006-8993(95)00407-h.
Fisher 344 rats were fed either ad libitum or with a diet containing a 40% reduction of calories beginning at 4 months of age. At 14 months and 22 months male rats were tested for their ability to learn a complex motor skill. At both ages the diet restricted rats reached criterion of performing 10 successful crosses in 10 min at an earlier time than ad libitum fed controls. At 22 months of age the diet restricted rats showed improved acquisition of running times for the task. Male rats at 14 and 22 months and female rats at 24 months were examined electrophysiologically for the ability of isoproterenol to augment the action of GABA in the cerebellum when both substances were applied iontophoretically from an extracellular multibarreled glass electrode. In all 3 age and sex groups there was an improvement in the beta-adrenergic receptor modulation of GABA responses in the dietary restricted vs. ad libitum rats. However, no difference was observed between dietary restricted and ad libitum rats when the number and affinity of cerebellar beta-adrenergic receptors was assessed with 125I-iodopindolol binding. Overall, there was a significant improvement in cerebellar noradrenergic function in the dietary restricted rats and this was accompanied by an improvement in motor learning.
将 Fisher 344 大鼠从 4 个月大开始随意喂食或喂食热量减少 40% 的饮食。在 14 个月和 22 个月时，对雄性大鼠学习复杂运动技能的能力进行测试。在这两个年龄段，饮食受限的大鼠比随意喂食的对照组更早达到在 10 分钟内成功穿越 10 次的标准。在 22 个月大时，饮食受限的大鼠在该任务的跑步时间获取方面表现出改善。对 14 个月和 22 个月的雄性大鼠以及 24 个月的雌性大鼠进行电生理检查，当从细胞外多管玻璃电极通过离子电渗法施加异丙肾上腺素和 GABA 时，检测异丙肾上腺素增强小脑 GABA 作用的能力。在所有 3 个年龄和性别组中，饮食受限的大鼠与随意喂食的大鼠相比，GABA 反应的β-肾上腺素能受体调节有所改善。然而，当用 125I-碘吲哚洛尔结合法评估小脑β-肾上腺素能受体的数量和亲和力时，饮食受限的大鼠与随意喂食的大鼠之间未观察到差异。总体而言，饮食受限的大鼠小脑去甲肾上腺素能功能有显著改善，同时运动学习也有所改善。
