汉坦病毒汉城病毒株SR-11与汉滩病毒株76-118在新生小鼠中的毒力比较。

Comparison of virulence between Seoul virus strain SR-11 and Hantaan virus strain 76-118 of hantaviruses in newborn mice.

作者信息

Yoo Y C, Yoshimatsu K, Yoshida R, Tamura M, Azuma I, Arikawa J

机构信息

Institute of Immunological Science, Hokkaido University, Japan.

出版信息

Microbiol Immunol. 1993;37(7):557-62. doi: 10.1111/j.1348-0421.1993.tb01677.x.

DOI:10.1111/j.1348-0421.1993.tb01677.x

PMID:7901740

Abstract

Virulence of hantavirus strain of SR-11 Seoul virus and Hantaan 76-118 (HTN) of Hantaan virus were compared. Infections of both strains were lethal in newborn mice. However, inoculum required to cause lethal infection was about 4,000 times higher for strain HTN (1.65 x 10(3) PFU/mouse/LD50) than for strain SR-11 (0.36 PFU). Thus, both strains were considered pathogenic to newborn mice but they possessed different levels of virulence. The assay system used for these strains in newborn mice proved to be useful in the study of hantavirus virulence. Growth curves of the two strains in CV-7 cell cultures were compared. Strain SR-11 was shown to have higher activity of virus replication and virus release into the culture fluids than strain HTN. The possibility of a relationship between replication activity and high levels of virulence in mice was suggested.

摘要

对汉城病毒SR - 11株和汉坦病毒汉滩76 - 118（HTN）株的毒力进行了比较。两种毒株感染新生小鼠均具有致死性。然而，引起致死性感染所需的接种量，HTN株（1.65×10³ PFU/小鼠/LD50）比SR - 11株（0.36 PFU）高约4000倍。因此，两种毒株均被认为对新生小鼠具有致病性，但它们的毒力水平不同。用于这些毒株在新生小鼠中的检测系统被证明在汉坦病毒毒力研究中是有用的。比较了两种毒株在CV - 7细胞培养物中的生长曲线。结果显示，SR - 11株比HTN株具有更高的病毒复制活性和向培养液中释放病毒的活性。这提示了在小鼠中复制活性与高毒力水平之间存在关联的可能性。