Watanabe S, Osumi M, Ohnishi T, Ichikawa E, Takahashi H
Department of Dermatology, Teikyo University School of Medicine, Tokyo, Japan.
Histochem Cell Biol. 1995 Jun;103(6):425-33. doi: 10.1007/BF01457542.
In order to investigate the re-epithelialization process during wound healing, the hair on the back of guinea pigs was shaved and then excisional wounds were made through the entire thickness of the skin. Histological changes were observed and changes in the expression of different cytokeratin polypeptides were examined using an immunohistochemical technique. Immunohistochemical study revealed that the proliferating and migrating keratinocytes expressed the same cytokeratins as the basal cells of normal epidermis. In addition, the entire epidermis of fairly remote areas from the edges of the wound where no thickening was observed showed a temporarily abnormal staining pattern. The suprabasal cells in the regenerating epidermis temporarily expressed cytokeratins not only specific for suprabasal cells but also specific for basal cells. The cytokeratins expressed in normal basal keratinocytes were also present in the thickened granular layers. These data indicate that the expression of cytokeratins in the epidermal keratinocytes (even in fairly remote areas from the wound edges) changes during wound healing, that the origin of the migrating keratinocytes from the remaining epidermis seems to be the basal cells in the epidermis, and that the appearance of keratohyalin granules is not related to changes in cytokeratin expression.
为了研究伤口愈合过程中的再上皮化过程，将豚鼠背部的毛发剃除，然后全层切除皮肤造成伤口。观察组织学变化，并使用免疫组织化学技术检测不同细胞角蛋白多肽表达的变化。免疫组织化学研究显示，增殖和迁移的角质形成细胞表达与正常表皮基底细胞相同的细胞角蛋白。此外，在伤口边缘相当远的区域，未观察到增厚的整个表皮显示出暂时异常的染色模式。再生表皮中的基底上层细胞不仅暂时表达基底上层细胞特有的细胞角蛋白，还表达基底细胞特有的细胞角蛋白。正常基底角质形成细胞中表达的细胞角蛋白也存在于增厚的颗粒层中。这些数据表明，在伤口愈合过程中，表皮角质形成细胞（即使在离伤口边缘相当远的区域）中的细胞角蛋白表达会发生变化，迁移的角质形成细胞似乎来源于表皮中的基底细胞，并且透明角质颗粒的出现与细胞角蛋白表达的变化无关。
