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抗人中间丝蛋白的单克隆抗体。II. 丝蛋白在正常人体组织中的分布。

Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues.

作者信息

Gown A M, Vogel A M

出版信息

Am J Pathol. 1984 Feb;114(2):309-21.

PMID:6320650
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1900342/
Abstract

Monoclonal antibodies generated against different human intermediate filament (IF) proteins were assayed on fixed, embedded tissue by the biotin-avidin-immunoperoxidase method for evaluation of the tissue specificity of these antibodies. An antibody (43 beta E8) made to fibroblast IF protein stains mesenchymal tissue such as endothelium, histiocytes, stromal fibroblasts, and Schwann cells but does not stain epithelium, skeletal muscle, lymphocytes, or neurons. Three different anti-cytokeratin antibodies decorate epithelium in three unique patterns. One (35 beta H11) stains all nonsquamous epithelium but fails to recognize squamous epithelium. Antibody 34 beta E12 stains the full thickness of squamous epithelium and ductular epithelium but does not react with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands. Antibody 34 beta B4 stains only the suprabasal portion of squamous epithelium. None of these three anti-cytokeratin antibodies reacts with nerve or mesenchymal tissue. Two anti-neurofilament antibodies recognize only neurons, failing to react with epithelial or mesenchymal tissue. We conclude that these anti-intermediate filament antibodies can be used as tissue-specific markers. Neoplasms retain the same intermediate filament patterns as the normal parental tissue; therefore, these antibodies can be used as diagnostic aids in surgical pathology.

摘要

采用生物素-抗生物素蛋白-免疫过氧化物酶法，在固定、包埋的组织上检测针对不同人类中间丝（IF）蛋白产生的单克隆抗体，以评估这些抗体的组织特异性。一种针对成纤维细胞IF蛋白产生的抗体（43βE8）可使间充质组织如内皮细胞、组织细胞、基质成纤维细胞和施万细胞染色，但不能使上皮细胞、骨骼肌、淋巴细胞或神经元染色。三种不同的抗细胞角蛋白抗体以三种独特的模式使上皮细胞染色。一种（35βH11）可使所有非鳞状上皮细胞染色，但不能识别鳞状上皮细胞。抗体34βE12可使鳞状上皮细胞和导管上皮细胞全层染色，但不与肝细胞、胰腺腺泡细胞、近端肾小管或子宫内膜腺反应。抗体34βB4仅使鳞状上皮细胞的基底上层部分染色。这三种抗细胞角蛋白抗体均不与神经组织或间充质组织反应。两种抗神经丝抗体仅识别神经元，不与上皮组织或间充质组织反应。我们得出结论，这些抗中间丝抗体可作为组织特异性标志物。肿瘤保留与正常亲代组织相同的中间丝模式；因此，这些抗体可作为外科病理学中的诊断辅助工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/26c8f315c618/amjpathol00185-0138-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/d7bdafa2469d/amjpathol00185-0135-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/aafac8ccd769/amjpathol00185-0137-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/26c8f315c618/amjpathol00185-0138-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/4aed608af0db/amjpathol00185-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/7e4f150175f6/amjpathol00185-0129-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/1e1bc4cb0d8e/amjpathol00185-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/536eacb3a270/amjpathol00185-0131-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/000f42b410c0/amjpathol00185-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/acdf56fd6fb2/amjpathol00185-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/773ae4e5046e/amjpathol00185-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/70261dccd42a/amjpathol00185-0134-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/d7bdafa2469d/amjpathol00185-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/fc2b688eacfa/amjpathol00185-0135-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/072308198d7e/amjpathol00185-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/aafac8ccd769/amjpathol00185-0137-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd7/1900342/26c8f315c618/amjpathol00185-0138-a.jpg

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