Vasdev S, Whalen M, Ford C A, Longerich L, Prabhakaran V, Parai S
Department of Medicine and Laboratory Medicine, General Hospital, St John's, Newfoundland.
Can J Cardiol. 1995 Oct;11(9):807-15.
To investigate the effects of oral L-threonine and ethanol, precursor of endogenous acetaldehyde, on systolic blood pressure, cystolic free calcium ([Ca2+]i) and vascular calcium uptake in Wistar-Kyoto (WKY) rats.
Twenty-four male WKY rats aged eight weeks were divided into four groups of six animals each. Animals were given either water or 5% ethanol, 8% L-threonine or 8% L-glycine in drinking water for 15 weeks, animals were sacrificed, aortic rings were incubated in physiological buffer containing 45Ca2+ and uptake was measured after 20 mins. ([Ca2+]i in platelets was measured with a fluorescence [Ca2+]i indicator, FURA-2. Tissues were processed for morphological investigation.
After 15 weeks, systolic blood pressure, platelet [Ca2+]i and aortic calcium uptake were all significantly higher (P < 0.001) in rats given either threonine or ethanol than in control rats given water or glycine. Animals in threonine or ethanol group also showed smooth muscle cell hyperplasia, with some thickening of the wall and narrowing of the lumen in small arteries and arterioles of the kidney. Glycine treatment did not cause any of these changes in rats.
These results suggest that acetaldehyde may be a common cause of both ethanol- and threonine-induced hypertension.
研究内源性乙醛的前体——口服L-苏氨酸和乙醇对Wistar-Kyoto（WKY）大鼠收缩压、胞质游离钙（[Ca2+]i）及血管钙摄取的影响。
将24只8周龄雄性WKY大鼠分为4组，每组6只。分别给予动物饮用含5%乙醇、8%L-苏氨酸或8%L-甘氨酸的水或普通水，持续15周。之后处死动物，将主动脉环置于含45Ca2+的生理缓冲液中孵育20分钟后测量钙摄取量。用荧光[Ca2+]i指示剂FURA-2测量血小板中的[Ca2+]i。对组织进行形态学研究。
15周后，给予苏氨酸或乙醇的大鼠收缩压、血小板[Ca2+]i和主动脉钙摄取量均显著高于给予水或甘氨酸的对照大鼠（P<0.001）。苏氨酸或乙醇组的动物还出现平滑肌细胞增生，肾小动脉和微动脉的管壁增厚，管腔狭窄。甘氨酸处理未引起大鼠出现上述任何变化。
这些结果表明，乙醛可能是乙醇和苏氨酸诱导高血压的共同原因。
