Ledesma E, Camaró M L, Carbonell E, Sacristán T, Martí A, Pellicer S, Llorca J, Herrero P, Dasí M A
Laboratorios Real, C. E. Durviz, S. L., Valencia, Spain.
Can J Microbiol. 1995 Sep;41(9):846-8. doi: 10.1139/m95-116.
Arbitrarily primed polymerase chain reaction (AP-PCR) was used to differentiate strains of Legionella pneumophila isolated from different water sources in a resort hotel in Benidorm, Alicante, Spain, where an outbreak of Legionnaires' disease occurred among a group of tourists between 65 and 80 years of age. All isolates were L. pneumophila serogroup 1, subtype Pontiac (Knoxville 1). Five different patterns (P1 to P5) were obtained by AP-PCR. The number of bands per pattern varied between 4 and 11. Patterns P1 and P2 represented 60 and 20% of L. pneumophila isolates, respectively. Since different subpopulations of L. pneumophila coexisted (up to three different AP-PCR patterns were identified in a single room), it was not possible to link an individual L. pneumophila strain to the occurrence of this outbreak.
采用任意引物聚合酶链反应（AP-PCR）对从西班牙阿利坎特贝尼多姆一家度假酒店不同水源分离出的嗜肺军团菌菌株进行鉴别，该酒店一群65至80岁的游客中发生了军团病暴发。所有分离株均为嗜肺军团菌血清型1，庞蒂亚克亚型（诺克斯维尔1型）。通过AP-PCR获得了五种不同的模式（P1至P5）。每种模式的条带数量在4至11条之间变化。模式P1和P2分别占嗜肺军团菌分离株的60%和20%。由于嗜肺军团菌的不同亚群共存（在单个房间中鉴定出多达三种不同的AP-PCR模式），因此无法将单个嗜肺军团菌菌株与此次暴发联系起来。
