运用随机引物PCR对医院获得性嗜肺军团菌菌株进行分子分型
Molecular typing of nosocomial strains of Legionella pneumophila by arbitrarily primed PCR.
作者信息
Grattard F, Berthelot P, Reyrolle M, Ros A, Etienne J, Pozzetto B
机构信息
Laboratoire de Bactériologie-Virologie, Faculté de Médecine J. Lisfranc, Saint-Etienne, France.
出版信息
J Clin Microbiol. 1996 Jun;34(6):1595-8. doi: 10.1128/jcm.34.6.1595-1598.1996.
Abstract
Arbitrarily primed PCR with two different primers was compared with ribotyping and monoclonal antibody analysis for typing Legionella strains. Applied to 11 epidemiologically unrelated strains, arbitrarily primed PCR resulted in an index of discrimination of 100% with both primers. It was found able to identify an epidemic clone of Legionella pneumophila serogroup 1 that was isolated from both patients and a hot water circuit of the same hospital.
摘要
采用两种不同引物的任意引物PCR技术与核糖体分型及单克隆抗体分析技术对嗜肺军团菌菌株进行分型比较。将任意引物PCR技术应用于11株流行病学上无关联的菌株，两种引物的鉴别指数均达100%。结果发现该技术能够鉴定出从同一医院的患者和热水循环系统中分离出的嗜肺军团菌血清型1的流行克隆。
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本文引用的文献
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