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本文引用的文献

1
Comparison of Legionella pneumophila isolates by arbitrarily primed PCR and pulsed-field gel electrophoresis: analysis from seven epidemic investigations.用任意引物PCR和脉冲场凝胶电泳对嗜肺军团菌分离株进行比较：来自七次疫情调查的分析
J Clin Microbiol. 1995 Nov;33(11):2872-5. doi: 10.1128/jcm.33.11.2872-2875.1995.
2
Comparison of arbitrarily primed polymerase chain reaction, ribotyping, and monoclonal antibody analysis for subtyping Legionella pneumophila serogroup 1.嗜肺军团菌血清1型分型的任意引物聚合酶链反应、核糖体分型和单克隆抗体分析比较
J Clin Microbiol. 1993 Jul;31(7):1940-2. doi: 10.1128/jcm.31.7.1940-1942.1993.
3
Typing of Legionella pneumophila strains by polymerase chain reaction-mediated DNA fingerprinting.通过聚合酶链反应介导的DNA指纹图谱对嗜肺军团菌菌株进行分型。
J Clin Microbiol. 1993 Aug;31(8):2198-200. doi: 10.1128/jcm.31.8.2198-2200.1993.
4
Arbitrarily primed PCR, ribotyping, and plasmid pattern analysis applied to investigation of a nosocomial outbreak due to Enterobacter cloacae in a neonatal intensive care unit.应用任意引物聚合酶链反应、核糖体分型和质粒图谱分析调查新生儿重症监护病房中由阴沟肠杆菌引起的医院感染暴发。
J Clin Microbiol. 1994 Mar;32(3):596-602. doi: 10.1128/jcm.32.3.596-602.1994.
5
Differentiation of Pseudomonas aeruginosa strains by ribotyping: high discriminatory power by using a single restriction endonuclease.通过核糖体分型对铜绿假单胞菌菌株进行鉴别：使用单一限制性内切酶具有高鉴别力。
J Med Microbiol. 1994 Apr;40(4):275-81. doi: 10.1099/00222615-40-4-275.
6
Legionella pneumonia in transplant recipients: a cluster of cases of eight years' duration.移植受者中的军团菌肺炎：持续八年的一组病例
J Hosp Infect. 1994 Mar;26(3):191-202. doi: 10.1016/0195-6701(94)90042-6.
7
Molecular fingerprinting of Legionella species by repetitive element PCR.通过重复元件PCR对军团菌进行分子指纹分析。
J Clin Microbiol. 1994 Dec;32(12):2989-94. doi: 10.1128/jcm.32.12.2989-2994.1994.
8
Characterization of nosocomial strains of Enterobacter aerogenes by arbitrarily primed-PCR analysis and ribotyping.通过任意引物聚合酶链反应分析和核糖体分型对产气肠杆菌医院菌株进行特征分析。
Infect Control Hosp Epidemiol. 1995 Apr;16(4):224-30. doi: 10.1086/647094.
9
Restriction fragment length polymorphism of rRNA genes for molecular typing of members of the family Legionellaceae.用于军团菌科成员分子分型的rRNA基因限制性片段长度多态性
J Clin Microbiol. 1995 Feb;33(2):402-6. doi: 10.1128/jcm.33.2.402-406.1995.
10
FK506 inhibits the differentiation of developing thymocytes but not negative selection of T cell receptor V beta 5+ and V beta 11+ T lymphocytes in vivo.FK506在体内可抑制发育中的胸腺细胞分化，但不影响T细胞受体Vβ5 +和Vβ11 + T淋巴细胞的阴性选择。
Transpl Immunol. 1994;2(1):11-21. doi: 10.1016/0966-3274(94)90072-8.

运用随机引物PCR对医院获得性嗜肺军团菌菌株进行分子分型

Molecular typing of nosocomial strains of Legionella pneumophila by arbitrarily primed PCR.

作者信息

Grattard F, Berthelot P, Reyrolle M, Ros A, Etienne J, Pozzetto B

机构信息

Laboratoire de Bactériologie-Virologie, Faculté de Médecine J. Lisfranc, Saint-Etienne, France.

出版信息

J Clin Microbiol. 1996 Jun;34(6):1595-8. doi: 10.1128/jcm.34.6.1595-1598.1996.

DOI:10.1128/jcm.34.6.1595-1598.1996
PMID:8735131
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229075/
Abstract

Arbitrarily primed PCR with two different primers was compared with ribotyping and monoclonal antibody analysis for typing Legionella strains. Applied to 11 epidemiologically unrelated strains, arbitrarily primed PCR resulted in an index of discrimination of 100% with both primers. It was found able to identify an epidemic clone of Legionella pneumophila serogroup 1 that was isolated from both patients and a hot water circuit of the same hospital.

摘要

采用两种不同引物的任意引物PCR技术与核糖体分型及单克隆抗体分析技术对嗜肺军团菌菌株进行分型比较。将任意引物PCR技术应用于11株流行病学上无关联的菌株，两种引物的鉴别指数均达100%。结果发现该技术能够鉴定出从同一医院的患者和热水循环系统中分离出的嗜肺军团菌血清型1的流行克隆。