Pollard M, Luckert P H, Scheu J
Lobund Laboratory, University of Notre Dame, IN 46556, USA.
Carcinogenesis. 1995 Oct;16(10):2419-21. doi: 10.1093/carcin/16.10.2419.
Aged Lobund-Wistar (L-W) rats develop: (i) spontaneous and induced metastasizing adenocarcinomas in the prostate and seminal vesicle (P-SV) complex; and (ii) spontaneous hepatomas and hepatocarcinomas. Within the time-frame of 14 months, similar adenocarcinomas were induced in the P-SV complex in 70-90% of younger L-W rats by a single i.v. inoculation of methylnitrosourea (MNU) which was followed by slow release s.c. implants of testosterone propionate (TP). Within the same time-frame, neither MNU nor TP alone induced significant incidences of P-SV tumors; and untreated control L-W rats were disease-free. Methylnitrosourea or TP and combinations thereof did not induce liver tumors. However, when MNU-inoculated L-W rats were fed phenobarbital (PB), they developed (i) metastasizing adenocarcinomas in the P-SV complex and (ii) altered cellular foci and nodules in the livers. Methylnitrosourea induced a high incidence of benign lung adenomas which progressed to lung cancers in numbers which were of marginal significance. Thus, dormant MNU-initiated cells in the P-SV complex were activated by phenobarbital, to produce adenocarcinomas in that complex.
老龄Lobund-Wistar（L-W）大鼠会出现：（i）前列腺和精囊（P-SV）复合体中的自发性和诱发性转移性腺癌；以及（ii）自发性肝癌和肝细胞癌。在14个月的时间范围内，通过单次静脉注射甲基亚硝基脲（MNU），随后皮下植入丙酸睾酮（TP）缓释剂，70-90%的年轻L-W大鼠的P-SV复合体中会诱发类似的腺癌。在同一时间范围内，单独使用MNU或TP都不会诱发P-SV肿瘤的显著发病率；未治疗的对照L-W大鼠没有疾病。甲基亚硝基脲或TP及其组合不会诱发肝肿瘤。然而，当给接种了MNU的L-W大鼠喂食苯巴比妥（PB）时，它们会出现：（i）P-SV复合体中的转移性腺癌，以及（ii）肝脏中细胞灶和结节的改变。甲基亚硝基脲诱发了高发病率的良性肺腺瘤，其中进展为肺癌的数量具有边缘意义。因此，P-SV复合体中潜伏的由MNU启动的细胞被苯巴比妥激活，从而在该复合体中产生腺癌。
