Van Laer L, Vingerhoets J, Vanham G, Kestens L, Bwayo J, Otido J, Piot P, Roggen E
Department of Infection and Immunity, Institute of Tropical Medicine, Antwerp, Belgium.
Clin Exp Immunol. 1995 Nov;102(2):243-50. doi: 10.1111/j.1365-2249.1995.tb03772.x.
The cellular immune responses to fractionated Haemophilus ducreyi antigens, coated on latex beads, were assessed in patients with chancroid and in controls, using an in vitro lymphocyte proliferation assay. Several fractions of H. ducreyi antigen revealed stimulating activity. However, only the molecular size ranges 91-78 kD, 59-29 kD, and 25-21 kD induced proliferation that may be specifically related to H. ducreyi infection. Lymphocytes from four HIV- patients, successfully treated for chancroid, were not stimulated by H. ducreyi antigen. In general, lymphocytes from HIV+ chancroid patients were less responsive to H. ducreyi antigen compared with those from HIV- chancroid patients. However, two HIV-infected patients showed exceptionally strong responses to high molecular weight fractions. To our knowledge this is the first report demonstrating that H. ducreyi contains specific T cell-stimulating antigens. Based on this work, further identification and purification of the T cell antigens is feasible.
采用体外淋巴细胞增殖试验，对软下疳患者和对照组受试者针对包被在乳胶珠上的杜克雷嗜血杆菌分级抗原的细胞免疫反应进行了评估。杜克雷嗜血杆菌抗原的几个级分显示出刺激活性。然而，只有分子量范围为91 - 78 kD、59 - 29 kD和25 - 21 kD的级分诱导的增殖可能与杜克雷嗜血杆菌感染有特异性关联。4例成功治愈软下疳的HIV阴性患者的淋巴细胞未受到杜克雷嗜血杆菌抗原的刺激。总体而言，与HIV阴性软下疳患者相比，HIV阳性软下疳患者的淋巴细胞对杜克雷嗜血杆菌抗原的反应性较低。然而，两名HIV感染患者对高分子量级分表现出异常强烈的反应。据我们所知，这是首份证明杜克雷嗜血杆菌含有特异性T细胞刺激抗原的报告。基于这项工作，进一步鉴定和纯化T细胞抗原是可行的。
