Hussain L A, Kelly C G, Rodin A, Jourdan M, Lehner T
Department of Immunology, United Medical School, Guy's Hospital, London, UK.
Clin Exp Immunol. 1995 Nov;102(2):384-8. doi: 10.1111/j.1365-2249.1995.tb03794.x.
Rectal and cervicovaginal mucosa are common routes of transmission of HIV, although the mechanism of transmission is unknown. We have investigated human rectal and cervicovaginal epithelia for the expression of complement receptors (CR) and cell adhesion molecules which may be involved in HIV and other infections. In rectal mucosa, CR3 was detected in the surface and crypt epithelial cells by immunohistology, using MoAbs to CD18 and CD11b in 10 out of 15 specimens. RNA transcripts encoding both CD11b and CD18 were also demonstrated in surface and crypt epithelial cells by in situ hybridization. Although CD11b was detected in the epithelial cells in three out of the 14 cervicovaginal specimens, we were unable to detect CD18. We suggest that expression of the CD11b/CD18 heterodimer might facilitate transmission of HIV by enhancing binding of HIV-antibody complexes in seminal fluid to epithelial cells. Alternatively, since intercellular adhesion molecule-1 (ICAM-1) is a receptor for CD11b/CD18, this may promote adhesion between epithelial cells and HIV-infected mononuclear cells in seminal fluid.
直肠和宫颈阴道黏膜是HIV常见的传播途径，但其传播机制尚不清楚。我们研究了人类直肠和宫颈阴道上皮细胞中补体受体（CR）和细胞黏附分子的表达情况，这些分子可能与HIV及其他感染有关。在直肠黏膜中，通过免疫组织化学法，使用抗CD18和CD11b的单克隆抗体，在15个标本中的10个标本的表面和隐窝上皮细胞中检测到了CR3。通过原位杂交也在表面和隐窝上皮细胞中证实了编码CD11b和CD18的RNA转录本。虽然在14个宫颈阴道标本中的3个标本的上皮细胞中检测到了CD11b，但我们未能检测到CD18。我们认为，CD11b/CD18异二聚体的表达可能通过增强精液中HIV-抗体复合物与上皮细胞的结合来促进HIV的传播。另外，由于细胞间黏附分子-1（ICAM-1）是CD11b/CD18的受体，这可能会促进上皮细胞与精液中HIV感染的单核细胞之间的黏附。
