Tuffal G, Ponthus C, Picard C, Rivière M, Puzo G
Laboratoire de Pharmacologie et de Toxicologie Fondamentales du Centre National de la Recherche Scientifique, Toulouse, France.
Eur J Biochem. 1995 Oct 1;233(1):377-83. doi: 10.1111/j.1432-1033.1995.377_1.x.
The structures of methylglucose-containing polysaccharides (MeGlc PS) from Mycobacterium xenopi were investigated using high-pH anion-exchange chromatography and liquid secondary-ion mass spectrometry. We report the structure of two novel MeGlc PS, referred to as A and B. MeGlc PS A is composed of 16 D-glucopyranose residues, 11 of which are methylated, and MeGlc PS B contains 15 D-glucopyranose residues, 10 of which are methylated. The main structural feature of both MeGlc PS A and B, compared to the previously described structures, is the absence of the tetrasaccharide non-reducing end 3-O-Me-D-Glcp-[alpha(1-->4)-D-Glcp]3. The MeGlc PS A structure is similar to the synthetic polysaccharide [Saïer, M. H. & Ballou, C. E. (1968) J. Biol. Chem. 243, 992-1005], having a lower affinity for fatty acids than the MeGlc PS of Mycobacterium smegmatis [Kiho, T. & Ballou, C. E. (1988) Biochemistry 27, 5824-5828]. Thus, the occurrence of MeGlc PS A and the consequences on the regulation of fatty acid synthetase I activity involved in the biosynthesis of fatty acids, precursors of mycolic acid biosynthesis, is discussed.
利用高pH值阴离子交换色谱法和液体二次离子质谱法对来自偶然分枝杆菌的含甲基葡萄糖多糖（MeGlc PS）的结构进行了研究。我们报道了两种新型MeGlc PS（分别称为A和B）的结构。MeGlc PS A由16个D-吡喃葡萄糖残基组成，其中11个被甲基化，而MeGlc PS B含有15个D-吡喃葡萄糖残基，其中10个被甲基化。与先前描述的结构相比，MeGlc PS A和B的主要结构特征是不存在四糖非还原端3-O-Me-D-Glcp-[α(1→4)-D-Glcp]3。MeGlc PS A的结构与合成多糖[Saïer, M. H. & Ballou, C. E. (1968) J. Biol. Chem. 243, 992 - 1005]相似，对脂肪酸的亲和力低于耻垢分枝杆菌的MeGlc PS [Kiho, T. & Ballou, C. E. (1988) Biochemistry 27, 5824 - 5828]。因此，本文讨论了MeGlc PS A的存在情况以及其对参与脂肪酸生物合成（分枝菌酸生物合成前体）的脂肪酸合成酶I活性调节的影响。
