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1
Efficacy of direct Gram stain in differentiating staphylococci from streptococci in blood cultures positive for gram-positive cocci.直接革兰氏染色在革兰氏阳性球菌血培养阳性中鉴别葡萄球菌与链球菌的效能。
J Clin Microbiol. 1978 Feb;7(2):111-3. doi: 10.1128/jcm.7.2.111-113.1978.
2
Evaluation of rapid identification of gram-positive cocci in positive blood cultures by use of the AutoMicrobic system Gram-Positive Identification Card.使用自动微生物系统革兰氏阳性鉴定卡对血培养阳性标本中革兰氏阳性球菌进行快速鉴定的评估。
J Clin Microbiol. 1984 Aug;20(2):171-4. doi: 10.1128/jcm.20.2.171-174.1984.
3
Evaluation of several commercial biochemical and immunologic methods for rapid identification of gram-positive cocci directly from blood cultures.评估几种用于直接从血培养物中快速鉴定革兰氏阳性球菌的商业生化和免疫方法。
J Clin Microbiol. 1988 Jul;26(7):1335-8. doi: 10.1128/jcm.26.7.1335-1338.1988.
4
Development of a treatment algorithm for streptococci and enterococci from positive blood cultures identified with the Verigene Gram-positive blood culture assay.应用 Verigene 革兰阳性血培养仪鉴定阳性血培养中链球菌属和肠球菌属的治疗算法的制定。
J Clin Microbiol. 2013 Nov;51(11):3869-71. doi: 10.1128/JCM.01587-13. Epub 2013 Aug 28.
5
[Refinement of presumptive antimicrobial therapy based on initial microbiological information on positive blood culture].基于血培养阳性的初始微生物学信息对经验性抗菌治疗进行优化
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Fluorescence in situ hybridisation (FISH) accelerates identification of Gram-positive cocci in positive blood cultures.荧光原位杂交(FISH)可加速阳性血培养物中革兰氏阳性球菌的鉴定。
Int J Antimicrob Agents. 2008 Nov;32 Suppl 1:S51-9. doi: 10.1016/j.ijantimicag.2008.06.007. Epub 2008 Aug 20.
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[Identification of staphylococci directly from positive blood culture bottles by MALDI-TOF MS system].[通过基质辅助激光解吸电离飞行时间质谱系统直接从阳性血培养瓶中鉴定葡萄球菌]
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Rapid immunodiagnosis of streptococci and enterococci in blood cultures.血培养中链球菌和肠球菌的快速免疫诊断
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Gram stain interpretation of blood cultures.血培养的革兰氏染色结果判读
Clin Pediatr (Phila). 1982 Aug;21(8):463-5. doi: 10.1177/000992288202100803.
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Evaluation of the LightCycler Staphylococcus M GRADE kits on positive blood cultures that contained gram-positive cocci in clusters.对含有成簇革兰氏阳性球菌的阳性血培养物进行LightCycler金黄色葡萄球菌M GRADE试剂盒评估。
J Clin Microbiol. 2005 Dec;43(12):6144-6. doi: 10.1128/JCM.43.12.6144-6146.2005.

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Fluorescence Imaging of with the agglutinin (HPA) As a Potential, Rapid Diagnostic Tool.以血凝素(HPA)作为潜在的快速诊断工具对[具体内容缺失]进行荧光成像。
Front Microbiol. 2017 Jul 18;8:1333. doi: 10.3389/fmicb.2017.01333. eCollection 2017.
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First notification of positive blood cultures and the high accuracy of the gram stain report.血培养阳性的首次报告及革兰氏染色报告的高准确性。
J Clin Microbiol. 2007 Apr;45(4):1113-7. doi: 10.1128/JCM.02523-06. Epub 2007 Feb 14.
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Rapid identification of Staphylococcus aureus from BacT/ALERT blood culture bottles by direct Gram stain characteristics.通过直接革兰氏染色特征从BacT/ALERT血培养瓶中快速鉴定金黄色葡萄球菌。
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Identification of Staphylococcus species in blood cultures by a rapid commercial DNA probe test.通过快速商业DNA探针检测法鉴定血培养中的葡萄球菌属菌种。
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5
Multicenter evaluation of a modified protocol for the RAPIDEC staph system for direct identification of Staphylococcus aureus in blood cultures.用于血培养中直接鉴定金黄色葡萄球菌的RAPIDEC葡萄球菌系统改良方案的多中心评估。
J Clin Microbiol. 1998 Dec;36(12):3707-9. doi: 10.1128/JCM.36.12.3707-3709.1998.
6
Comparison of commercial slide agglutination kits with a tube coagulase test for the rapid identification of Staphylococcus aureus from blood culture.商业玻片凝集试剂盒与试管凝固酶试验用于从血培养物中快速鉴定金黄色葡萄球菌的比较。
J Clin Pathol. 1997 Feb;50(2):164-6. doi: 10.1136/jcp.50.2.164.
7
Rapid identification of Staphylococcus aureus in blood cultures by thermonuclease testing.通过热核酸酶检测快速鉴定血培养中的金黄色葡萄球菌。
J Clin Microbiol. 1983 Sep;18(3):722-4. doi: 10.1128/jcm.18.3.722-724.1983.
8
Direct identification of bacterial isolates in blood cultures by using a DNA probe.使用DNA探针直接鉴定血培养中的细菌分离株。
J Clin Microbiol. 1991 Oct;29(10):2193-6. doi: 10.1128/jcm.29.10.2193-2196.1991.
9
Comparison of direct and standard antimicrobial disk susceptibility testing for bacteria isolated from blood.从血液中分离出的细菌的直接抗菌药敏试验与标准抗菌药敏试验的比较。
J Clin Microbiol. 1979 Oct;10(4):482-7. doi: 10.1128/jcm.10.4.482-487.1979.

本文引用的文献

1
Comparison of macroscopic examination, routine gram stains, and routine subcultures in the initial detection of positive blood cultures.在血培养阳性的初始检测中,宏观检查、常规革兰氏染色和常规传代培养的比较。
Appl Microbiol. 1974 Mar;27(3):537-9. doi: 10.1128/am.27.3.537-539.1974.
2
Susceptibility of anaerobic bacteria to 23 antimicrobial agents.厌氧菌对23种抗菌剂的敏感性。
Antimicrob Agents Chemother. 1976 Oct;10(4):736-52. doi: 10.1128/AAC.10.4.736.

直接革兰氏染色在革兰氏阳性球菌血培养阳性中鉴别葡萄球菌与链球菌的效能。

Efficacy of direct Gram stain in differentiating staphylococci from streptococci in blood cultures positive for gram-positive cocci.

作者信息

Agger W A, Maki D G

出版信息

J Clin Microbiol. 1978 Feb;7(2):111-3. doi: 10.1128/jcm.7.2.111-113.1978.

DOI:10.1128/jcm.7.2.111-113.1978
PMID:75888
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274874/
Abstract

A preponderance of clusters seen on direct Gram stain of blood cultures positive for gram-positive cocci was 98% sensitive and 100% specific for identification of staphylococcal species or of Peptococcus. A preponderance of chains, pairs, or both was 100% sensitive and 98% specific for identifying streptococci. Further presumptive identification of either staphylococci or streptococci based on microscopic morphology was unreliable. The direct Gram stain is highly reliable for differentiating staphylococci from streptococci and should be of considerable value to clinicians selecting initial antimicrobial therapy.

摘要

在革兰氏阳性球菌血培养直接革兰氏染色中观察到的大量菌簇,对于鉴定葡萄球菌属或消化球菌属,敏感度为98%,特异度为100%。大量的链状、成对或两者皆有的形态,对于鉴定链球菌的敏感度为100%,特异度为98%。基于显微镜形态对葡萄球菌或链球菌进行进一步的初步鉴定并不可靠。直接革兰氏染色在区分葡萄球菌和链球菌方面高度可靠,对于临床医生选择初始抗菌治疗应具有相当大的价值。