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通过直接革兰氏染色特征从BacT/ALERT血培养瓶中快速鉴定金黄色葡萄球菌。

Rapid identification of Staphylococcus aureus from BacT/ALERT blood culture bottles by direct Gram stain characteristics.

作者信息

Murdoch D R, Greenlees R L

机构信息

Microbiology Unit, Canterbury Health Laboratories, Christchurch 8001, New Zealand.

出版信息

J Clin Pathol. 2004 Feb;57(2):199-201. doi: 10.1136/jcp.2003.10538.

DOI:10.1136/jcp.2003.10538
PMID:14747451
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1770202/
Abstract

The rapid identification of Staphylococcus aureus from positive blood cultures provides important clinical and therapeutic information. Using criteria based on direct Gram stain characteristics, an experienced microscopist was able to distinguish S aureus from other staphylococci isolated from BacT/ALERT blood culture bottles with an overall sensitivity of 89% and specificity of 98%. Furthermore, this method was readily taught to a clinical microbiologist who had not previously used the method first hand. Laboratories using the BacT/ALERT blood culture system should become familiar with these criteria so that S aureus bacteraemia can be identified rapidly.

摘要

从阳性血培养物中快速鉴定金黄色葡萄球菌可提供重要的临床和治疗信息。根据直接革兰氏染色特征制定标准,经验丰富的显微镜检查人员能够从BacT/ALERT血培养瓶中分离出的其他葡萄球菌中区分出金黄色葡萄球菌,总体灵敏度为89%,特异性为98%。此外,该方法很容易传授给一位此前未曾亲自使用过该方法的临床微生物学家。使用BacT/ALERT血培养系统的实验室应熟悉这些标准,以便能够快速鉴定金黄色葡萄球菌菌血症。

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本文引用的文献

1
Direct identification of Staphylococcus aureus from positive blood culture bottles.从阳性血培养瓶中直接鉴定金黄色葡萄球菌。
J Clin Microbiol. 2003 Feb;41(2):889-91. doi: 10.1128/JCM.41.2.889-891.2003.
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Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system.利用LightCycler系统从BacT/ALERT血培养瓶中快速鉴定金黄色葡萄球菌和mecA基因。
J Clin Microbiol. 2002 Jul;40(7):2659-61. doi: 10.1128/JCM.40.7.2659-2661.2002.
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Evaluation of four methods for rapid identification of Staphylococcus aureus from blood cultures.四种从血培养物中快速鉴定金黄色葡萄球菌方法的评估
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Efficacy of direct Gram stain in differentiating staphylococci from streptococci in blood cultures positive for gram-positive cocci.直接革兰氏染色在革兰氏阳性球菌血培养阳性中鉴别葡萄球菌与链球菌的效能。
J Clin Microbiol. 1978 Feb;7(2):111-3. doi: 10.1128/jcm.7.2.111-113.1978.