在血培养阳性的初始检测中，宏观检查、常规革兰氏染色和常规传代培养的比较。
Comparison of macroscopic examination, routine gram stains, and routine subcultures in the initial detection of positive blood cultures.
作者信息
Blazevic D J, Stemper J E, Matsen J M
出版信息
Appl Microbiol. 1974 Mar;27(3):537-9. doi: 10.1128/am.27.3.537-539.1974.
Abstract
Blood was cultured in two vaccum bottles containing Columbia broth with sodium polyanethol sulfonate and CO(2). Filtered air was admitted to one bottle, and the bottles were incubated at 35 C until growth was detected or for a maximum of 7 days. Bottles were examined daily for macroscopic growth. Gram stains were made routinely on the 1st, 4th, and 7th days, and samples were routinely subcultured to sheep blood agar (incubated in GasPak jar) and chocolate agar (incubated in CO(2)) on the 1st and 4th days of incubation. Of 1,127 positive blood cultures, 65% were first detected by macroscopic examination, 23% were first detected by Gram stain, and 12% were first detected only by subculture.
摘要
血液接种于两个含有添加多聚茴香脑磺酸钠的哥伦比亚肉汤并充入二氧化碳的真空瓶中。向其中一个瓶中通入过滤空气，将瓶子置于35℃孵育，直至检测到生长或最长孵育7天。每天检查瓶子有无肉眼可见的生长。常规在第1天、第4天和第7天进行革兰氏染色，并在孵育的第1天和第4天常规将样本接种到羊血琼脂（在厌氧罐中孵育）和巧克力琼脂（在二氧化碳环境中孵育）上。在1127份阳性血培养物中，65%首先通过肉眼检查检测到，23%首先通过革兰氏染色检测到，12%仅通过传代培养首次检测到。
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