Larinkari U, Rautio M
Laboratory of Clinical Microbiology, Kymenlaakso Central Hospital, Kotka, Finland.
Eur J Clin Microbiol Infect Dis. 1995 Jul;14(7):606-9. doi: 10.1007/BF01690735.
A selective medium was incorporated into a new three-media dipslide (Uricult Trio, Orion Diagnostica) to allow rapid identification of Escherichia coli. The medium is supplemented with a recently described chromogenic substrate, hydroxyquinoline-beta-D-glucuronide, for beta-glucuronidase enzyme. The performance of the medium was compared to that of three other beta-glucuronidase detection methods in tests of 602 routine urine samples. Of 324 Escherichia coli strains isolated, 92% grew brown colonies on dipslide, thus being beta-glucuronidase positive. The proportion of beta-glucuronidase-positive Escherichia coli detected by the three methods was 93% for BGA II agar plates (Tammer-Tutka), 91% for PGUA tablets (Rosco) and 84% for Fluorocult Brolacin agar plates (Merck). No false-positive reactions were seen in the case of 209 significant isolates of species other than Escherichia coli grown on the selective medium.
一种选择性培养基被整合到一种新型的三介质浸片（尿酸检测三联片，奥立安诊断公司）中，以实现对大肠杆菌的快速鉴定。该培养基添加了一种最近描述的用于β-葡萄糖醛酸酶的显色底物——羟基喹啉-β-D-葡萄糖醛酸。在对602份常规尿液样本的检测中，将该培养基的性能与其他三种β-葡萄糖醛酸酶检测方法进行了比较。在分离出的324株大肠杆菌菌株中，92%在浸片上生长出棕色菌落，因此为β-葡萄糖醛酸酶阳性。三种方法检测到的β-葡萄糖醛酸酶阳性大肠杆菌比例分别为：BGA II琼脂平板（坦默-图特卡）为93%，PGUA片剂（罗斯科）为91%，氟化物培养溴拉辛琼脂平板（默克）为84%。在选择性培养基上生长的209株非大肠杆菌的重要分离株未出现假阳性反应。
