Kämpfer P, Rauhoff O, Dott W
Fachgebiet Hygiene, Technische Universität Berlin, Federal Republic of Germany.
J Clin Microbiol. 1991 Dec;29(12):2877-9. doi: 10.1128/jcm.29.12.2877-2879.1991.
A total of 712 strains representing 47 taxa of the family Enterobacteriaceae were tested for the ability to hydrolyze 14 4-methylumbelliferyl (4-MU)-linked substrates within 3 h of incubation. In addition to the well-known differentiation potential of the hydrolysis of 4-MU-beta-D-galactopyranoside, 4-MU-beta-D-glucuronide, and 4-MU-beta-D-xylopyranoside, the hydrolysis of some other fluorogenic substrates (e.g., 4-MU-beta-D-fucopyranoside, 4-MU-N-acetyl-beta-D-galactosaminide, and 4-MU-alpha-D-galactopyranoside) can also be used for species differentiation within the family Enterobacteriaceae.
对代表肠杆菌科47个分类单元的712株菌株进行了测试,以检测它们在培养3小时内水解14种4-甲基伞形酮(4-MU)连接底物的能力。除了4-MU-β-D-吡喃半乳糖苷、4-MU-β-D-葡萄糖醛酸苷和4-MU-β-D-吡喃木糖苷水解的众所周知的鉴别潜力外,一些其他荧光底物(如4-MU-β-D-吡喃岩藻糖苷、4-MU-N-乙酰-β-D-半乳糖胺和4-MU-α-D-吡喃半乳糖苷)的水解也可用于肠杆菌科内的菌种鉴别。