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Rapid detection of Escherichia coli in urine samples by a new chromogenic beta-glucuronidase assay.

作者信息

Delisle G J, Ley A

机构信息

Department of Microbiology, Hotel Dieu Hospital, Kingston, Ontario, Canada.

出版信息

J Clin Microbiol. 1989 Apr;27(4):778-9. doi: 10.1128/jcm.27.4.778-779.1989.

DOI:10.1128/jcm.27.4.778-779.1989
PMID:2656750
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267418/
Abstract

A new compound, indoxyl-beta-D-glucuronide, was assessed as a substrate for the rapid detection of Escherichia coli in urine. Incorporation of this compound into MacConkey agar allowed the direct differentiation of E. coli as deep blue colonies distinct from lactose and nonlactose fermenters. The sensitivity was 88 to 90%, and the specificity was 100%.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f46/267418/c2b11e416029/jcm00064-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f46/267418/c2b11e416029/jcm00064-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f46/267418/c2b11e416029/jcm00064-0196-a.jpg

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本文引用的文献

1
PRESENTATION, DIAGNOSIS, AND TREATMENT OF URINARY-TRACT INFECTIONS IN GENERAL PRACTICE.一般实践中尿路感染的表现、诊断及治疗
Lancet. 1965 Mar 6;1(7384):514-6. doi: 10.1016/s0140-6736(65)92020-9.
2
Fluorogenic assays for immediate confirmation of Escherichia coli.用于立即确认大肠杆菌的荧光测定法。
Appl Environ Microbiol. 1982 Jun;43(6):1320-9. doi: 10.1128/aem.43.6.1320-1329.1982.
3
Survival and growth characteristics of Escherichia coli associated with hemorrhagic colitis.与出血性结肠炎相关的大肠杆菌的存活及生长特性
对DipStreak的评估，这是一种具有原创划线机制的新设备，用于检测、计数和初步鉴定尿路病原体。
J Clin Microbiol. 2002 Jun;40(6):2169-75. doi: 10.1128/JCM.40.6.2169-2178.2002.
4
Cost-effective and rapid presumptive identification of gram-negative bacilli in routine urine, pus, and stool cultures: evaluation of the use of CHROMagar orientation medium in conjunction with simple biochemical tests.在常规尿液、脓液和粪便培养物中对革兰氏阴性杆菌进行经济高效且快速的初步鉴定：评估CHROMagar定向培养基与简单生化试验联合使用的情况。
J Clin Microbiol. 2000 Dec;38(12):4586-92. doi: 10.1128/JCM.38.12.4586-4592.2000.
5
Evaluation of BBL CHROMagar orientation medium for detection and presumptive identification of urinary tract pathogens.评估BBL CHROMagar定向培养基用于检测和初步鉴定尿路病原体的效果。
J Clin Microbiol. 1997 Nov;35(11):2773-7. doi: 10.1128/jcm.35.11.2773-2777.1997.
6
Evaluation of CPS ID2 medium for diagnosis of urinary infections.用于诊断泌尿系统感染的CPS ID2培养基的评估
Eur J Clin Microbiol Infect Dis. 1995 Dec;14(12):1111-3. doi: 10.1007/BF01590953.
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