嗜热栖热菌5S rRNA结构域E的结晶及初步衍射研究
Crystallization and preliminary diffraction studies of the structural domain E of Thermus flavus 5S rRNA.
作者信息
Nolte A, Klussman S, Lorenz S, Bald R, Betzel C, Dauter Z, Wilson K, Fürste J P, Erdmann V A
机构信息
Institut für Biochemie, Freie Universität Berlin, Germany.
出版信息
FEBS Lett. 1995 Oct 30;374(2):292-4. doi: 10.1016/0014-5793(95)01136-3.
The ribosomal 5S RNA is an essential constituent of the large ribosomal subunit. To overcome the difficulties of crystallizing large RNA molecules such as 5S rRNAs, we decided to divide the 5S rRNA in five domains A through E to determine their structure. Recently we determined the crystal structural of the helical domain A. Here we report the crystallization of the chemically synthesized domain E of the Thermus flavus 5S rRNA. The crystal form is trigonal with unit cell dimensions: a = b = 42.80 A and c = 162.20 A. Diffraction-data to 2.8 A have been recorded and the structure solution is currently underway by means of MIR and MAD techniques.
核糖体5S RNA是核糖体大亚基的重要组成部分。为了克服诸如5S rRNA等大型RNA分子结晶的困难，我们决定将5S rRNA划分为A至E五个结构域以确定其结构。最近我们确定了螺旋结构域A的晶体结构。在此我们报告嗜热栖热菌5S rRNA化学合成结构域E的结晶情况。晶体形态为三方晶系，晶胞参数：a = b = 42.80 Å，c = 162.20 Å。已记录了分辨率达2.8 Å的衍射数据，目前正通过多波长反常散射（MIR）和多波长反常色散（MAD）技术进行结构解析。
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