Betzel C, Lorenz S, Fürste J P, Bald R, Zhang M, Schneider T R, Wilson K S, Erdmann V A
Institut für Biochemie, Freie Universität Berlin, Germany.
FEBS Lett. 1994 Sep 5;351(2):159-64. doi: 10.1016/0014-5793(94)00834-5.
This is the first high resolution crystal structure of an RNA molecule made by solid phase chemical synthesis and representing a natural RNA. The structure of the domain A of Thermus flavus ribosomal 5S RNA is refined to R = 18% at 2.4 A including 159 solvent molecules. Most of the 2'-hydroxyl groups as well as the phosphate oxygens are involved either in specific hydrogen bonds in intermolecular contacts or to solvent molecules. The two U-G and G-U base-pairs are stabilized by H-bonds supplied via three water molecules to compensate for the lack of base-pair hydrogen bonds. The structure shows for the first time in detail the importance of highly ordered internal water in stabilizing an RNA structure.
这是通过固相化学合成制备的、代表天然RNA的RNA分子的首个高分辨率晶体结构。嗜热栖热菌核糖体5S RNA的结构域A的结构在2.4 Å分辨率下精修至R = 18%，包含159个溶剂分子。大多数2'-羟基以及磷酸氧原子要么参与分子间接触中的特定氢键形成，要么与溶剂分子相互作用。两个U-G和G-U碱基对通过三个水分子提供的氢键得以稳定，以弥补碱基对氢键的缺失。该结构首次详细展示了高度有序的内部水在稳定RNA结构中的重要性。
