苦艾对乙酰氨基酚和四氯化碳诱导的肝毒性的预防和治疗作用。

Preventive and curative effects of Artemisia absinthium on acetaminophen and CCl4-induced hepatotoxicity.

Gilani A H, Janbaz K H

Department of Pharmacology, Aga Khan University Medical College, Faculty of Health Sciences, Karachi, Pakistan.

Gen Pharmacol. 1995 Mar;26(2):309-15. doi: 10.1016/0306-3623(94)00194-r.

Abstract

Effect of aqueous-methanolic extract of Artemisia absinthium (Compositae) was investigated against acetaminophen- and CCl4-induced hepatic damage. 2. Acetaminophen produced 100% mortality at the dose of 1 g/kg in mice while pretreatment of animals with plant extract (500 mg/kg) reduced the death rate to 20%. 3. Pretreatment of rats with plant extract (500 mg/kg, orally twice daily for two days) prevented (P < 0.01) the acetaminophen (640 mg/kg) as well as CCl4 (1.5 ml/kg)-induced rise in serum transaminases (GOT and GPT). 4. Post-treatment with three successive doses of extract (500 mg/kg, 6 hr) restricted the hepatic damage induced by acetaminophen (P < 0.01) but CCl4-induced hepatotoxicity was not altered (P > 0.05). 5. Plant extract (500 mg/kg) caused significant prolongation (P < 0.05) in pentobarbital (75 mg/kg)-induced sleep as well as increased strychnine-induced lethality in mice suggestive of inhibitory effect on microsomal drug metabolizing enzymes (MDME). 6. These results indicate that the crude extract of Artemisia absinthium exhibits hepatoprotective action partly through MDME inhibitory action and validates the traditional use of plant in hepatic damage.

摘要

研究了苦艾（菊科）水甲醇提取物对乙酰氨基酚和四氯化碳诱导的肝损伤的作用。2. 乙酰氨基酚在小鼠中剂量为1 g/kg时导致100%死亡率，而用植物提取物（500 mg/kg）预处理动物可将死亡率降至20%。3. 用植物提取物（500 mg/kg，口服，每日两次，持续两天）预处理大鼠可预防（P < 0.01）乙酰氨基酚（640 mg/kg）以及四氯化碳（1.5 ml/kg）诱导的血清转氨酶（谷草转氨酶和谷丙转氨酶）升高。4. 连续三次给予提取物（500 mg/kg，间隔6小时）进行治疗后，可限制乙酰氨基酚诱导的肝损伤（P < 0.01），但四氯化碳诱导的肝毒性未改变（P > 0.05）。5. 植物提取物（500 mg/kg）可显著延长（P < 0.05）戊巴比妥（75 mg/kg）诱导的睡眠时间，并增加士的宁对小鼠的致死率，提示对微粒体药物代谢酶（MDME）有抑制作用。6. 这些结果表明，苦艾粗提取物部分通过抑制MDME发挥肝保护作用，并证实了该植物在肝损伤方面的传统用途。