Mizrahi V, Huberts P, Dawes S S, Dudding L R
Molecular Biology Unit, South African Institute for Medical Research, Johannesburg.
Gene. 1993 Dec 22;136(1-2):287-90. doi: 10.1016/0378-1119(93)90481-h.
Internal segments of the gyrA and polA genes involved in DNA replication of Mycobacterium tuberculosis and rnhA of M. smegmatis, have been amplified by the polymerase chain reaction (PCR) using degenerate oligodeoxyribonucleotide primers based on conserved sequences. The deduced amino acid sequences were 54-66% homologous to the corresponding segments of their Escherichia coli counterparts. This method provides a useful means of cloning genes encoding DNA replication enzymes of mycobacteria.
利用基于保守序列的简并寡脱氧核糖核苷酸引物，通过聚合酶链反应（PCR）扩增了结核分枝杆菌DNA复制相关的gyrA和polA基因的内部片段，以及耻垢分枝杆菌的rnhA基因。推导的氨基酸序列与它们大肠杆菌对应物的相应片段具有54%-66%的同源性。该方法为克隆编码分枝杆菌DNA复制酶的基因提供了一种有用的手段。
