经聚合酶链反应确认的杜克雷嗜血杆菌无症状携带情况。
Asymptomatic carriage of Haemophilus ducreyi confirmed by the polymerase chain reaction.
作者信息
Hawkes S, West B, Wilson S, Whittle H, Mabey D
机构信息
Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, UK.
出版信息
Genitourin Med. 1995 Aug;71(4):224-7. doi: 10.1136/sti.71.4.224.
Abstract
OBJECTIVE
To investigate asymptomatic carriage of Haemophilus ducreyi by using polymerase chain reaction (PCR) on samples from women at high risk of infection.
SUBJECTS
213 commercial sex workers (CSWs) recruited in The Gambia, West Africa.
METHODS
Genital samples (cervical, vaginal and ulcer) were tested for the presence of H ducreyi by PCR with the technique of "one tube nested primer".
RESULTS
12 CSWs were PCR positive for H ducreyi; 8 of these women had genital ulcers on examination.
CONCLUSION
Using a simplified PCR technique for detecting H ducreyi we have shown that 2% of CSWs were carrying the organism without clinical symptoms or signs. This has important implications for sexually transmitted disease control programmes in areas with a high prevalence of chancroid.
摘要
目的
通过对感染高危女性的样本进行聚合酶链反应（PCR）来调查杜克雷嗜血杆菌的无症状携带情况。
对象
在西非冈比亚招募的213名商业性工作者（CSW）。
方法
采用“一管巢式引物”技术，通过PCR检测生殖器样本（宫颈、阴道和溃疡处样本）中是否存在杜克雷嗜血杆菌。
结果
12名CSW的杜克雷嗜血杆菌PCR检测呈阳性；其中8名女性经检查有生殖器溃疡。
结论
使用简化的PCR技术检测杜克雷嗜血杆菌，我们发现2%的CSW携带该病原体但无临床症状或体征。这对软下疳高发地区的性传播疾病控制项目具有重要意义。
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