Boury N M, Czuprynski C J
Department of Medical Microbiology and Immunology, University of Wisconsin-Madison 53706, USA.
Immunol Lett. 1995 May;46(1-2):111-6. doi: 10.1016/0165-2478(95)00027-3.
Several studies have reported that Listeria monocytogenes multiples within hepatocytes and that inflammatory neutrophils inhibit this intracellular growth in vivo. In the present study, we used a murine embryonic hepatocyte cell line (ATCC TIB73) as an in vitro model to investigate neutrophil-hepatocyte interactions. Murine peritoneal exudate neutrophils adhered more readily to L. monocytogenes-infected hepatocyte monolayers than to uninfected monolayers or monolayers infected with actA- and hly- mutants of L. monocytogenes. L. monocytogenes-infected TIB73 cells increased their surface expression of ICAM-1 as compared with uninfected TIB73 cells. Neutrophil adherence and oxidative stress to TIB73 cells were reduced by pre-incubating the hepatocyte monolayers with anti-ICAM-1 monoclonal antibody and diminished further by pre-incubating the peritoneal exudate neutrophils with an anti-CR3 monoclonal antibody.
多项研究报告称，单核细胞增生李斯特菌在肝细胞内增殖，且炎症中性粒细胞在体内可抑制这种细胞内生长。在本研究中，我们使用小鼠胚胎肝细胞系（ATCC TIB73）作为体外模型来研究中性粒细胞与肝细胞的相互作用。与未感染的单层细胞或感染了单核细胞增生李斯特菌actA和hly突变体的单层细胞相比，小鼠腹腔渗出液中性粒细胞更容易黏附于感染单核细胞增生李斯特菌的肝细胞单层细胞。与未感染的TIB73细胞相比，感染单核细胞增生李斯特菌的TIB73细胞ICAM-1的表面表达增加。用抗ICAM-1单克隆抗体预孵育肝细胞单层细胞可减少中性粒细胞对TIB73细胞的黏附和氧化应激，而用抗CR3单克隆抗体预孵育腹腔渗出液中性粒细胞可进一步降低这种黏附和氧化应激。
