基于聚合酶链式反应检测束状菌毛基因鉴定肠致病性大肠杆菌
Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene.
作者信息
Gunzburg S T, Tornieporth N G, Riley L W
机构信息
Department of Medicine, Cornell University Medical College, New York, New York 10021, USA.
出版信息
J Clin Microbiol. 1995 May;33(5):1375-7. doi: 10.1128/jcm.33.5.1375-1377.1995.
Abstract
A rapid and simple method of detecting enteropathogenic Escherichia coli (EPEC) was developed. The procedure is based on amplifying by the PCR method a 326-bp region of the bundle-forming pilus gene of EPEC. The oligonucleotide DNA primers used in this procedure did not amplify DNA of any other bacterial enteropathogens tested. The procedure was 100% specific for EPEC strains that exhibit a characteristic pattern of attachment (localized adherence) to HeLa cells.
摘要
开发了一种快速简便的检测肠致病性大肠杆菌（EPEC）的方法。该方法基于通过PCR方法扩增EPEC束状菌毛基因的一个326bp区域。此方法中使用的寡核苷酸DNA引物不会扩增所检测的任何其他细菌性肠道病原体的DNA。该方法对表现出对HeLa细胞具有特征性附着模式（局部黏附）的EPEC菌株具有100%的特异性。
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