Wright A M, Hoxey E V, Soper C J, Davies D J
School of Pharmacy and Pharmacology, University of Bath, UK.
J Appl Bacteriol. 1995 Oct;79(4):432-8. doi: 10.1111/j.1365-2672.1995.tb03158.x.
Preliminary screening was carried out on spores of 29 strains of Bacillus stearothermophilus to determine their potential as biological indicator organisms for low temperature steam and formaldehyde sterilization. Each strain was sporulated on four chemically defined media. Fourteen strains produced satisfactory sporulation on one or more of the media but there was considerable variation in the extent of sporulation. The growth index of the spores, which was dependent on both the strain of organism and the sporulation medium, ranged from 1% to 90%. The spores were appraised on the basis of their resistance to inactivation by 0.5% w/v formaldehyde in aqueous solution at 70 degrees C. The survivor curves obtained could be characterized into five types on the basis of the shape of the curve. Only five strains of Bacillus stearothermophilus produced spores with the characteristics of high resistance, linear semi-logarithmic survivor curve and high growth index that would be required of a potential biological indicator organism.
对29株嗜热脂肪芽孢杆菌的孢子进行了初步筛选，以确定它们作为低温蒸汽甲醛灭菌生物指示菌的潜力。每株菌在四种化学成分明确的培养基上产孢。14株菌在一种或多种培养基上产生了令人满意的产孢效果，但产孢程度存在相当大的差异。孢子的生长指数取决于菌株和产孢培养基，范围为1%至90%。根据孢子在70℃下对0.5%（w/v）甲醛水溶液灭活的抗性对其进行评估。根据曲线形状，所获得的存活曲线可分为五种类型。只有五株嗜热脂肪芽孢杆菌产生的孢子具有作为潜在生物指示菌所需的高抗性、线性半对数存活曲线和高生长指数的特征。
