来自不同限制修饰系统的调控C蛋白可以相互互补。
The regulatory C proteins from different restriction-modification systems can cross-complement.
作者信息
Ives C L, Sohail A, Brooks J E
机构信息
New England Biolabs, Beverly, Massachusetts 01915, USA.
出版信息
J Bacteriol. 1995 Nov;177(21):6313-5. doi: 10.1128/jb.177.21.6313-6315.1995.
Abstract
The BamHI restriction-modification system contains a third gene, bamHIC, which positively regulates bamHIR. Similar small genes from other systems were tested in vivo for their ability to cross-complement. C.BamHI protein was identified, purified, and used to raise polyclonal antibodies. Attempts to detect other C proteins in cell extracts by cross-reactivity with C.BamHI antibodies proved unsuccessful.
摘要
BamHI限制修饰系统包含第三个基因bamHIC，它对bamHIR起正向调节作用。对来自其他系统的类似小基因进行了体内交叉互补能力测试。鉴定、纯化了C.BamHI蛋白，并用于制备多克隆抗体。试图通过与C.BamHI抗体的交叉反应在细胞提取物中检测其他C蛋白，但未成功。
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