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2
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Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.位点特异性甲基化对限制性内切核酸酶和DNA修饰甲基转移酶的影响。
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本文引用的文献

1
A rapid method for acid hydrolysis of protein with a mixture of trifluoroacetic acid and hydrochloric acid.一种用三氟乙酸和盐酸混合物对蛋白质进行酸水解的快速方法。
Eur J Biochem. 1982 Jun;124(3):585-8. doi: 10.1111/j.1432-1033.1982.tb06634.x.
2
Codon catalog usage and the genome hypothesis.密码子目录使用与基因组假说。
Nucleic Acids Res. 1980 Jan 11;8(1):r49-r62. doi: 10.1093/nar/8.1.197-c.
3
Compilation and analysis of Escherichia coli promoter DNA sequences.大肠杆菌启动子DNA序列的汇编与分析
Nucleic Acids Res. 1983 Apr 25;11(8):2237-55. doi: 10.1093/nar/11.8.2237.
4
Expression of the Eco RI restriction-modification system and the construction of positive-selection cloning vectors.Eco RI限制修饰系统的表达及正选择克隆载体的构建。
Gene. 1982 Dec;20(2):219-29. doi: 10.1016/0378-1119(82)90041-5.
5
pUR222, a vector for cloning and rapid chemical sequencing of DNA.pUR222，一种用于DNA克隆和快速化学测序的载体。
Nucleic Acids Res. 1981 Aug 25;9(16):4087-98. doi: 10.1093/nar/9.16.4087.
6
Preliminary x-ray diffraction studies of EcoRI restriction endonuclease-DNA complex.大肠杆菌限制性内切酶EcoRI与DNA复合物的初步X射线衍射研究。
J Mol Biol. 1981 Jan 25;145(3):607-10. doi: 10.1016/0022-2836(81)90548-9.
7
Cloning and expression of the Pst I restriction-modification system in Escherichia coli.Pst I 限制修饰系统在大肠杆菌中的克隆与表达。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1503-7. doi: 10.1073/pnas.78.3.1503.
8
Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase.编码Eco RI核酸内切酶和甲基化酶的DNA序列分析。
J Biol Chem. 1981 Mar 10;256(5):2143-53.
9
DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes.Eco RI DNA限制酶和修饰酶结构基因的DNA序列。
J Biol Chem. 1981 Mar 10;256(5):2131-9.
10
Molecular cloning of seven mouse immunoglobulin kappa chain messenger ribonucleic acids.七种小鼠免疫球蛋白κ链信使核糖核酸的分子克隆
Biochemistry. 1980 Jun 10;19(12):2702-10. doi: 10.1021/bi00553a026.

大肠杆菌Eco RV限制与修饰系统编码基因的特性分析。

Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli.

作者信息

Bougueleret L, Schwarzstein M, Tsugita A, Zabeau M

出版信息

Nucleic Acids Res. 1984 Apr 25;12(8):3659-76. doi: 10.1093/nar/12.8.3659.

DOI:10.1093/nar/12.8.3659
PMID:6328432
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318777/
Abstract

A plasmid encoding the recently described Eco RV restriction and modification system has been isolated and characterized. This plasmid, pLB1 , is 6.2 kb long and carries only the Eco RV genes. A subclone of 3 kb has been inserted in pBR322. The relative positions of the endonuclease and the methylase genes were determined by the construction of a set of overlapping deletions generated by Bal31 resection. The DNA sequence of a 2.2 kb fragment containing the two genes was determined. The two genes are transcribed divergently from a 310 bp region and the assignment of the coding region has been confirmed by direct aminoacid sequence analysis. Possible mechanisms of regulation of the endonuclease gene expression at the translational level are proposed and discussed.

摘要

一种编码最近描述的Eco RV限制与修饰系统的质粒已被分离和鉴定。该质粒pLB1长6.2 kb，仅携带Eco RV基因。一个3 kb的亚克隆已插入pBR322中。通过Bal31切除产生的一组重叠缺失构建体确定了内切核酸酶和甲基化酶基因的相对位置。测定了包含这两个基因的2.2 kb片段的DNA序列。这两个基因从一个310 bp的区域双向转录，编码区的归属已通过直接氨基酸序列分析得到证实。提出并讨论了在翻译水平上内切核酸酶基因表达的可能调控机制。