大肠杆菌Eco RV限制与修饰系统编码基因的特性分析。
Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli.
作者信息
Bougueleret L, Schwarzstein M, Tsugita A, Zabeau M
出版信息
Nucleic Acids Res. 1984 Apr 25;12(8):3659-76. doi: 10.1093/nar/12.8.3659.
Abstract
A plasmid encoding the recently described Eco RV restriction and modification system has been isolated and characterized. This plasmid, pLB1 , is 6.2 kb long and carries only the Eco RV genes. A subclone of 3 kb has been inserted in pBR322. The relative positions of the endonuclease and the methylase genes were determined by the construction of a set of overlapping deletions generated by Bal31 resection. The DNA sequence of a 2.2 kb fragment containing the two genes was determined. The two genes are transcribed divergently from a 310 bp region and the assignment of the coding region has been confirmed by direct aminoacid sequence analysis. Possible mechanisms of regulation of the endonuclease gene expression at the translational level are proposed and discussed.
摘要
一种编码最近描述的Eco RV限制与修饰系统的质粒已被分离和鉴定。该质粒pLB1长6.2 kb，仅携带Eco RV基因。一个3 kb的亚克隆已插入pBR322中。通过Bal31切除产生的一组重叠缺失构建体确定了内切核酸酶和甲基化酶基因的相对位置。测定了包含这两个基因的2.2 kb片段的DNA序列。这两个基因从一个310 bp的区域双向转录，编码区的归属已通过直接氨基酸序列分析得到证实。提出并讨论了在翻译水平上内切核酸酶基因表达的可能调控机制。
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