Gilhar A, Zelickson B, Ulman Y, Etzioni A
Skin Research Laboratory, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
J Invest Dermatol. 1995 Nov;105(5):683-6. doi: 10.1111/1523-1747.ep12324456.
There are conflicting results regarding the role of autoantibodies in the pathogenesis of vitiligo. To examine their in vivo effect, human skin was transplanted onto nude mice injected with purified IgG obtained from patients with vitiligo and from controls. The effect was evaluated by several techniques. Dihydroxyphenylalanine staining revealed a marked decrease in the number of melanocytes in skin grafted onto mice injected with patients' IgG. Direct immunofluorescence staining demonstrated the presence of human IgG throughout the epidermis in specimens injected with purified IgG from vitiligo patients. No staining was observed when control IgG was injected. Electron microscopy studies demonstrated a marked decrease in melanin pigmentation with only rare melanosomes and melanocytes detected in grafts injected with patients' IgG. Thus all three techniques showed the destructive effect of vitiligo patients' serum on melanocytes. Our study highlights the important role of autoantibodies in the pathogenesis of vitiligo.
关于自身抗体在白癜风发病机制中的作用，存在相互矛盾的结果。为了研究它们在体内的作用，将人类皮肤移植到注射了从白癜风患者和对照组获得的纯化IgG的裸鼠身上。通过多种技术评估其效果。二羟基苯丙氨酸染色显示，移植到注射了患者IgG的小鼠身上的皮肤中黑素细胞数量显著减少。直接免疫荧光染色表明，在注射了白癜风患者纯化IgG的标本中，整个表皮都存在人IgG。注射对照IgG时未观察到染色。电子显微镜研究表明，注射患者IgG的移植物中黑色素沉着显著减少，仅检测到罕见的黑素小体和黑素细胞。因此，这三种技术均显示白癜风患者血清对黑素细胞具有破坏作用。我们的研究突出了自身抗体在白癜风发病机制中的重要作用。
