Tsuji T, Mimura Y, Wen S, Li X, Kanekawa A, Sasaki K, Shinozaki F
Department of Oral and Maxillofacial Surgery, Yamaguchi University School of Medicine, Ube.
Int J Oral Maxillofac Surg. 1995 Jun;24(3):221-5. doi: 10.1016/s0901-5027(06)80132-3.
The expression of PCNA and p53 protein was evaluated in a total of 75 cases of benign and malignant lesions of the oral cavity, comprising 50 squamous cell carcinomas (SCCs), 14 leukoplakias, and 11 pleomorphic adenomas. The DNA histogram of 20 SCCs was measured by flow cytometry. p53-positive cells were frequently seen in SCCs, but were rare in leukoplakias and pleomorphic adenomas. The PCNA labeling index (LI) was higher in SCCs than in other benign lesions. The expression rate of p53 protein was markedly elevated in SCCs obtained from smoking patients, when compared to nonsmoking patients. DNA ploidy did not show a close relationship with PCNA and p53 expression. The mean value of PCNA LI for 22 cases carrying positive p53 protein was 52.3%, which was higher than that of p53 protein negative cases (35.7%). The Kaplan-Meier survival curve of the patients who were negative for p53 was significantly more favorable than for patients who were positive for p53 (P < 0.01, Cox-Mantel test). These results suggest that PCNA and p53 LI are markers for the malignant potential of the oral mucosa, and are a useful indicator suggesting a poor prognosis.
共对75例口腔良恶性病变进行了PCNA和p53蛋白表达评估，其中包括50例鳞状细胞癌（SCC）、14例白斑和11例多形性腺瘤。采用流式细胞术对20例SCC的DNA直方图进行了测定。p53阳性细胞在SCC中常见，但在白斑和多形性腺瘤中少见。SCC的PCNA标记指数（LI）高于其他良性病变。与不吸烟患者相比，吸烟患者的SCC中p53蛋白表达率明显升高。DNA倍体与PCNA和p53表达无密切关系。22例p53蛋白阳性病例的PCNA LI平均值为52.3%，高于p53蛋白阴性病例（35.7%）。p53阴性患者的Kaplan-Meier生存曲线明显优于p53阳性患者（P < 0.01，Cox-Mantel检验）。这些结果表明，PCNA和p53 LI是口腔黏膜恶性潜能的标志物，是提示预后不良的有用指标。
