单核细胞穿越内皮细胞以及通过细胞外基质的过程涉及血小板内皮细胞黏附分子-1（PECAM-1）的不同分子结构域。
Migration of monocytes across endothelium and passage through extracellular matrix involve separate molecular domains of PECAM-1.
作者信息
Liao F, Huynh H K, Eiroa A, Greene T, Polizzi E, Muller W A
机构信息
Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York 10021-6399, USA.
出版信息
J Exp Med. 1995 Nov 1;182(5):1337-43. doi: 10.1084/jem.182.5.1337.
Abstract
During the inflammatory response, the adhesion molecule PECAM plays a crucial role in transendothelial migration, the passage of leukocytes across endothelium. We report here an additional role for PECAM in the subsequent migration of monocytes through the subendothelial extracellular matrix. PECAM has six immunoglobulin (Ig) superfamily domains. Monoclonal antibodies whose epitopes map to domains 1 and/or 2 selectively block monocyte migration through the endothelial junction, whereas those that map to domain 6 block only the migration through the extracellular matrix, trapping the monocyte between the endothelium and its basal lamina. Therefore, transendothelial migration (diapedesis) and passage through extracellular matrix (interstitial migration) are distinct and separable phases of monocyte emigration. Furthermore, distinct and separate Ig domains of PECAM are involved in mediating these two steps.
摘要
在炎症反应过程中，黏附分子PECAM在白细胞跨内皮迁移（即白细胞穿过内皮的过程）中起关键作用。我们在此报告PECAM在单核细胞随后穿过内皮下细胞外基质的迁移过程中的额外作用。PECAM有六个免疫球蛋白（Ig）超家族结构域。表位定位于结构域1和/或2的单克隆抗体可选择性地阻断单核细胞通过内皮连接的迁移，而表位定位于结构域6的单克隆抗体仅阻断通过细胞外基质的迁移，将单核细胞截留在内皮及其基膜之间。因此，跨内皮迁移（渗出）和穿过细胞外基质（间质迁移）是单核细胞外渗的不同且可分离的阶段。此外，PECAM不同且独立的Ig结构域参与介导这两个步骤。
相似文献
1
Migration of monocytes across endothelium and passage through extracellular matrix involve separate molecular domains of PECAM-1.单核细胞穿越内皮细胞以及通过细胞外基质的过程涉及血小板内皮细胞黏附分子-1（PECAM-1）的不同分子结构域。
J Exp Med. 1995 Nov 1;182(5):1337-43. doi: 10.1084/jem.182.5.1337.
2
Localization of multiple functional domains on human PECAM-1 (CD31) by monoclonal antibody epitope mapping.通过单克隆抗体表位作图对人PECAM-1（CD31）上多个功能结构域进行定位
Cell Adhes Commun. 1995 Feb;3(1):45-66. doi: 10.3109/15419069509081277.
3
PECAM-1 is required for transendothelial migration of leukocytes.血小板内皮细胞黏附分子-1（PECAM-1）是白细胞跨内皮迁移所必需的。
J Exp Med. 1993 Aug 1;178(2):449-60. doi: 10.1084/jem.178.2.449.
4
Individually distinct Ig homology domains in PECAM-1 regulate homophilic binding and modulate receptor affinity.血小板内皮细胞黏附分子-1（PECAM-1）中各自独特的免疫球蛋白（Ig）同源结构域调节同源性结合并调节受体亲和力。
J Biol Chem. 1996 May 10;271(19):11090-8. doi: 10.1074/jbc.271.19.11090.
5
Platelet endothelial cell adhesion molecule-1 (PECAM-1) homophilic adhesion is mediated by immunoglobulin-like domains 1 and 2 and depends on the cytoplasmic domain and the level of surface expression.血小板内皮细胞黏附分子-1（PECAM-1）的同源性黏附由免疫球蛋白样结构域1和2介导，并取决于胞质结构域和表面表达水平。
J Biol Chem. 1996 Aug 2;271(31):18561-70. doi: 10.1074/jbc.271.31.18561.
6
The use of anti-PECAM reagents in the control of inflammation.抗PECAM试剂在炎症控制中的应用。
Agents Actions Suppl. 1995;46:147-57. doi: 10.1007/978-3-0348-7276-8_15.
7
Identification of a novel transcript of human PECAM-1 and its role in the transendothelial migration of monocytes and Ca2+ mobilization.人PECAM-1新转录本的鉴定及其在单核细胞跨内皮迁移和Ca2+动员中的作用。
Biochem Biophys Res Commun. 2004 Aug 6;320(4):1228-35. doi: 10.1016/j.bbrc.2004.05.226.
8
The role of PECAM-1 (CD31) in leukocyte emigration: studies in vitro and in vivo.PECAM-1（CD31）在白细胞迁移中的作用：体内外研究
J Leukoc Biol. 1995 Apr;57(4):523-8. doi: 10.1002/jlb.57.4.523.
9
Soluble domain 1 of platelet-endothelial cell adhesion molecule (PECAM) is sufficient to block transendothelial migration in vitro and in vivo.血小板内皮细胞黏附分子（PECAM）的可溶性结构域1足以在体外和体内阻断跨内皮迁移。
J Exp Med. 1997 Apr 7;185(7):1349-57. doi: 10.1084/jem.185.7.1349.
10
Lipoxygenase metabolites induced expression of adhesion molecules and transendothelial migration of monocyte-like HL-60 cells is linked to protein kinase C activation.脂氧合酶代谢产物诱导黏附分子的表达以及单核细胞样HL-60细胞的跨内皮迁移与蛋白激酶C激活有关。
J Cell Physiol. 1996 Jun;167(3):477-87. doi: 10.1002/(SICI)1097-4652(199606)167:3<477::AID-JCP12>3.0.CO;2-1.
引用本文的文献
1
Complement System and Adhesion Molecule Skirmishes in Fabry Disease: Insights into Pathogenesis and Disease Mechanisms.补体系统与黏附分子在法布瑞氏病中的交锋：对发病机制和疾病机制的深入了解。
Int J Mol Sci. 2024 Nov 14;25(22):12252. doi: 10.3390/ijms252212252.
2
A human stem cell-derived model reveals pathologic extracellular matrix remodeling in diabetic podocyte injury.一种源自人类干细胞的模型揭示了糖尿病足细胞损伤中病理性细胞外基质重塑。
Matrix Biol Plus. 2024 Nov 2;24:100164. doi: 10.1016/j.mbplus.2024.100164. eCollection 2024 Dec.
3
Mechanotransduction via endothelial adhesion molecule CD31 initiates transmigration and reveals a role for VEGFR2 in diapedesis.通过内皮细胞黏附分子 CD31 进行机械转导启动了穿越，并揭示了 VEGFR2 在穿胞作用中的作用。
Immunity. 2023 Oct 10;56(10):2311-2324.e6. doi: 10.1016/j.immuni.2023.08.001. Epub 2023 Aug 28.
4
CD31 as a probable responding and gate-keeping protein of the blood-brain barrier and the risk of Alzheimer's disease.CD31 作为血脑屏障的可能反应和控制蛋白与阿尔茨海默病的风险。
J Cereb Blood Flow Metab. 2023 Jul;43(7):1027-1041. doi: 10.1177/0271678X231170041. Epub 2023 Apr 12.
5
Human CD99L2 Regulates a Unique Step in Leukocyte Transmigration.人 CD99L2 调控白细胞迁移中的独特步骤。
J Immunol. 2022 Sep 1;209(5):1001-1012. doi: 10.4049/jimmunol.2101091. Epub 2022 Aug 1.
6
Atomic Level Dissection of the Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1) Homophilic Binding Interface: Implications for Endothelial Cell Barrier Function.原子水平剖析血小板内皮细胞黏附分子 1（PECAM-1）同型结合界面：对内皮细胞屏障功能的影响。
Arterioscler Thromb Vasc Biol. 2022 Feb;42(2):193-204. doi: 10.1161/ATVBAHA.121.316668. Epub 2021 Dec 23.
7
The role of tumor necrosis factor-α and interferon-γ in the hyperglycemia-induced ubiquitination and loss of platelet endothelial cell adhesion molecule-1 in rat retinal endothelial cells.肿瘤坏死因子-α和干扰素-γ在高血糖诱导的大鼠视网膜内皮细胞中血小板内皮细胞黏附分子-1泛素化和丢失中的作用。
Microcirculation. 2021 Oct;28(7):e12717. doi: 10.1111/micc.12717. Epub 2021 May 29.
8
PECAM-1 supports leukocyte diapedesis by tension-dependent dephosphorylation of VE-cadherin.PECAM-1 通过 VE-钙黏蛋白的张力依赖性去磷酸化来支持白细胞的穿细胞运动。
EMBO J. 2021 May 3;40(9):e106113. doi: 10.15252/embj.2020106113. Epub 2021 Feb 19.
9
The glycocalyx: a central regulator of vascular function.糖萼：血管功能的中央调节者。
Am J Physiol Regul Integr Comp Physiol. 2021 Apr 1;320(4):R508-R518. doi: 10.1152/ajpregu.00340.2020. Epub 2021 Jan 27.
10
Endothelial Basement Membrane Laminins as an Environmental Cue in Monocyte Differentiation to Macrophages.内皮基底膜层粘连蛋白作为单核细胞向巨噬细胞分化的环境线索。
Front Immunol. 2020 Oct 30;11:584229. doi: 10.3389/fimmu.2020.584229. eCollection 2020.
本文引用的文献
1
Murine platelet endothelial cell adhesion molecule (PECAM-1)/CD31 modulates beta 2 integrins on lymphokine-activated killer cells.小鼠血小板内皮细胞黏附分子（PECAM-1）/CD31调节淋巴因子激活的杀伤细胞上的β2整合素。
Eur J Immunol. 1993 Oct;23(10):2464-71. doi: 10.1002/eji.1830231013.
2
PECAM-1 is required for transendothelial migration of leukocytes.血小板内皮细胞黏附分子-1（PECAM-1）是白细胞跨内皮迁移所必需的。
J Exp Med. 1993 Aug 1;178(2):449-60. doi: 10.1084/jem.178.2.449.
3
Platelet/endothelial cell adhesion molecule-1 (CD31)-mediated cellular aggregation involves cell surface glycosaminoglycans.血小板/内皮细胞黏附分子-1（CD31）介导的细胞聚集涉及细胞表面糖胺聚糖。
J Biol Chem. 1993 Jul 25;268(21):16037-46.
4
Deletions in the cytoplasmic domain of platelet-endothelial cell adhesion molecule-1 (PECAM-1, CD31) result in changes in ligand binding properties.血小板内皮细胞黏附分子-1（PECAM-1，CD31）胞质结构域的缺失导致配体结合特性的改变。
J Cell Biol. 1994 Jan;124(1-2):195-203. doi: 10.1083/jcb.124.1.195.
5
Involvement of platelet-endothelial cell adhesion molecule-1 in neutrophil recruitment in vivo.血小板内皮细胞黏附分子-1在体内中性粒细胞募集中的作用。
Science. 1993 Dec 3;262(5139):1580-2. doi: 10.1126/science.8248808.
6
Monoclonal antibody to murine PECAM-1 (CD31) blocks acute inflammation in vivo.抗小鼠PECAM-1（CD31）单克隆抗体可在体内阻断急性炎症。
J Exp Med. 1994 Mar 1;179(3):1059-64. doi: 10.1084/jem.179.3.1059.
7
8
Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1.α4β7整合素介导淋巴细胞与黏膜血管地址素MAdCAM-1的结合。
Cell. 1993 Jul 16;74(1):185-95. doi: 10.1016/0092-8674(93)90305-a.
9
Localization of multiple functional domains on human PECAM-1 (CD31) by monoclonal antibody epitope mapping.通过单克隆抗体表位作图对人PECAM-1（CD31）上多个功能结构域进行定位
Cell Adhes Commun. 1995 Feb;3(1):45-66. doi: 10.3109/15419069509081277.
10
Interactions of the chondroitin sulfate proteoglycan phosphacan, the extracellular domain of a receptor-type protein tyrosine phosphatase, with neurons, glia, and neural cell adhesion molecules.硫酸软骨素蛋白聚糖磷蛋白（一种受体型蛋白酪氨酸磷酸酶的细胞外结构域）与神经元、神经胶质细胞及神经细胞黏附分子的相互作用。
J Cell Biol. 1994 Dec;127(6 Pt 1):1703-15. doi: 10.1083/jcb.127.6.1703.
Zotero 插件