Kim C J, Shin K S, Kim W Y, Lim D S, Yoon S K, Park Y M, Kim B S, Jang S K, Cho M J
College of Veterinary Medicine, Chungnam National University, Taejon, Korea.
J Med Virol. 1995 Aug;46(4):380-6. doi: 10.1002/jmv.1890460415.
Comparative nucleotide sequence studies of the genomes of hepatitis C virus (HCV) revealed that there are at least 6 different genotypes of HCV. The prevalence of HCV genotypes among the patients with liver diseases in Korea was investigated using the polymerase chain reaction (PCR) for the NS5 region. In the 75 HCV RNA positive samples, two genotypes, type 1b and type 2a, were the major causative agents which accounted for 60% and 33% of infections respectively, while 7% could not be assigned a genotype by the methods used. The nucleotide sequences of cDNAs encoding the putative envelope proteins from 10 type 1b and 5 type 2a genotype samples were analyzed. Approximately 31-42% of the nucleotide sequences of type 1b samples examined differed from those of different genotypes. In the case of type 2a samples, 36-42% of the nucleotide sequences differed from those of different genotypes. The diversities of the amino acid sequences were the same or greater than those of the nucleotide sequences. Two hypervariable regions (HVR1 and HVR2) were recognized in both HCV genomes of genotypes 1b and 2a. However, the sequence divergence within the HVR2 region of genotype 2a was less than that of genotype 1b.
丙型肝炎病毒（HCV）基因组的比较核苷酸序列研究表明，HCV至少有6种不同的基因型。采用针对NS5区域的聚合酶链反应（PCR），对韩国肝病患者中HCV基因型的流行情况进行了调查。在75份HCV RNA阳性样本中，两种基因型，即1b型和2a型，是主要致病因子，分别占感染病例的60%和33%，而7%的样本无法通过所用方法确定其基因型。对来自10份1b型和5份2a型基因型样本的编码假定包膜蛋白的cDNA核苷酸序列进行了分析。所检测的1b型样本中，约31%-42%的核苷酸序列与不同基因型的序列不同。对于2a型样本，36%-42%的核苷酸序列与不同基因型的序列不同。氨基酸序列的多样性与核苷酸序列相同或更大。在1b型和2a型HCV基因组中均识别出两个高变区（HVR1和HVR2）。然而，2a型基因型HVR2区域内的序列差异小于1b型基因型。
