Wu R R, Mizokami M, Lau J Y, Ohno T, Fang Z X, Ohba K, Wu X S, Hata A, Sasaki M, Iino S
Department of Biochemistry, Nagoya City University Medical School, Japan.
J Med Virol. 1995 Feb;45(2):174-8. doi: 10.1002/jmv.1890450211.
The seroprevalence of hepatitis C virus (HCV) infection in Lanzhou, Western China was studied. HCV genotypes in 20 patients with HCV infection was determined by genotype-specific primer for polymerase chain reaction (PCR) based on HCV core region and compared with the genotype assigned by sequence comparison and molecular evolutionary analysis based on the same region. Antibody to HCV (anti-HCV) was present in 2.5% of volunteer blood donors and in 35.0% of paid blood donors (P < 0.01). HCV infection is uncommon in patients with liver disease who attended liver clinics in this locality; 4.0% with acute hepatitis and 4.0% with chronic hepatitis, 10.0% with liver cirrhosis, and none with hepatocellular carcinoma were seropositive for anti-HCV. Genotype 1b and 2a were both found to be prevalent. Together, they accounted for 19 of 20 (95%) patients with HCV infection. Sequencing of the HCV core region from two patients showed that the assignment of HCV genotype by genotype-specific primers for PCR matched well with the genotyping results based on sequence comparison and molecular evolutionary analysis. These data showed that HCV is present in Western China, HCV infection is more common in paid blood donors, and HCV genotypes 1b and 2a are both prevalent in Western China.
对中国西部兰州地区丙型肝炎病毒（HCV）感染的血清流行率进行了研究。采用基于HCV核心区域的聚合酶链反应（PCR）基因型特异性引物，对20例HCV感染患者的HCV基因型进行了测定，并与基于同一区域的序列比较和分子进化分析所确定的基因型进行了比较。抗HCV抗体在2.5%的无偿献血者和35.0%的有偿献血者中存在（P<0.01）。在该地区肝病门诊就诊的肝病患者中，HCV感染并不常见；急性肝炎患者中4.0%、慢性肝炎患者中4.0%、肝硬化患者中10.0%抗HCV血清学阳性，肝细胞癌患者中无一例抗HCV血清学阳性。发现1b型和2a型基因型均很普遍。它们一起占20例HCV感染患者中的19例（95%）。对两名患者的HCV核心区域进行测序显示，通过PCR基因型特异性引物对HCV基因型的判定与基于序列比较和分子进化分析的基因分型结果吻合良好。这些数据表明，HCV存在于中国西部，HCV感染在有偿献血者中更为常见，并且1b型和2a型HCV基因型在中国西部均很普遍。
