Govindan M, Govindan G N, Kingston D G
Division of Science and Mathematics, University of the Virgin Islands, St. Thomas 00802.
J Nat Prod. 1995 Aug;58(8):1174-84. doi: 10.1021/np50122a004.
As part of a collaborative research effort between the University of the Virgin Islands and Virginia Polytechnic Institute and State University, we carried out the extraction and bioassay of 87 marine organisms in a mechanism-based assay involving genetically altered yeast strains. Of these, nineteen showed differential activity between the mutant and wild-type yeast strains indicating the presence of potential DNA interacting agents. We now report the isolation and characterization of five new diterpenoids, 2-6, together with the previously known diterpenoid 1, from the bioactive extracts of the gorgonian Eunicea tourneforti forma atra. The structures of the isolated compounds were determined by employing a variety of one- and two-dimensional nmr methods.
作为维尔京群岛大学与弗吉尼亚理工学院暨州立大学合作研究工作的一部分，我们在一项基于机制的试验中，对87种海洋生物进行了提取和生物测定，该试验涉及基因改造的酵母菌株。其中，有19种在突变型和野生型酵母菌株之间表现出不同的活性，表明存在潜在的DNA相互作用剂。我们现在报告从柳珊瑚Eunicea tourneforti forma atra的生物活性提取物中分离并鉴定出5种新的二萜类化合物2 - 6，以及先前已知的二萜类化合物1。通过使用各种一维和二维核磁共振方法确定了分离出的化合物的结构。
