Rateb Mostafa E, Houssen Wael E, Schumacher Marc, Harrison William T A, Diederich Marc, Ebel Rainer, Jaspars Marcel
Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB243UE, Scotland, UK.
J Nat Prod. 2009 Aug;72(8):1471-6. doi: 10.1021/np900233c.
Three new compounds of the rare classes trisnorditerpenes, bisnorditerpenes, and norditerpenes, gracilins J-L (1, 2, 6), and a new diterpene, 3'-norspongiolactone (8), were isolated from the extract of the marine sponge Spongionella sp. using NMR- and bioassay-guided fractionation, in addition to three known gracilins (3-5) as well as the known diterpenoid tetrahydroaplysulphurin-1 (7). The structures were elucidated using NMR spectroscopic techniques and mass spectrometric analysis. The structure of gracilin H (3) was confirmed by single-crystal X-ray analysis. All compounds were tested for their cytotoxicity and for their potential to inhibit EGF-R tyrosine kinase.
从海洋海绵Spongionella sp.的提取物中，利用核磁共振和生物活性导向分级分离法，分离出了三种罕见类别的新化合物：三降二萜、双降二萜和降二萜，即细枝杉醇J-L（1、2、6），以及一种新的二萜3'-去甲海绵内酯（8），此外还分离出了三种已知的细枝杉醇（3-5）以及已知的二萜类化合物四氢阿朴硫磺素-1（7）。通过核磁共振光谱技术和质谱分析确定了这些化合物的结构。细枝杉醇H（3）的结构通过单晶X射线分析得以确证。对所有化合物进行了细胞毒性测试以及抑制表皮生长因子受体（EGF-R）酪氨酸激酶活性的潜力测试。
