Groenewald H B
Department of Anatomy, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
Onderstepoort J Vet Res. 1994 Sep;61(3):207-13.
Previous studies indicated large, thin-walled, milk-filled rumens in lethal grey and white Karakul lambs. There was also a significant decrease in the number and size of the myenteric plexuses and the number of ganglion cells in these lambs. The purpose of this study was to determine whether the myenteric ganglia of the affected lambs are functional, by testing for the presence of vaso-active intestinal peptide, somatostatin, neurotensin, neuropeptide Y, met-enkephalin, calcitonin gene-related peptide and substance P in the myenteric ganglia and nerve fibres in the forestomach and abomasum of grey, white and black Karakul lambs. Four 1-cm2 samples were taken from analogous areas of the wall of the rumen, reticulum, omasum and abomasum of five grey, five white and five black newborn Karakul lambs. They were pinned to wax squares, fixed for 18 h in Zamboni's fixative, dehydrated and rehydrated through graded alcohols and stored in phosphate-buffered saline. The outer longitudinal muscle layer of each sample of the rumen, reticulum, omasum and abomasum was separated from the rest of the tissue layers, stained for each of the seven neuropeptides by employment of the immunofluorescence technique, and studied with a Leitz Orthoplan fluorescent microscope. All the material studied tested positive for all the neuropeptides. It is concluded that all the peptides tested for were present in all the lambs and that the myenteric ganglia are therefore functional in the lethal lambs.
先前的研究表明，致死的灰色和白色卡拉库尔羔羊有大的、薄壁的、充满乳汁的瘤胃。这些羔羊的肌间神经丛数量和大小以及神经节细胞数量也显著减少。本研究的目的是通过检测灰色、白色和黑色卡拉库尔羔羊前胃和皱胃的肌间神经节和神经纤维中是否存在血管活性肠肽、生长抑素、神经降压素、神经肽Y、甲硫氨酸脑啡肽、降钙素基因相关肽和P物质，来确定患病羔羊的肌间神经节是否具有功能。从五只灰色、五只白色和五只黑色新生卡拉库尔羔羊的瘤胃、网胃、瓣胃和皱胃壁的类似区域采集四个1平方厘米的样本。将它们固定在蜡块上，用赞博尼固定液固定18小时，通过梯度酒精脱水和复水，然后保存在磷酸盐缓冲盐水中。将瘤胃、网胃、瓣胃和皱胃每个样本的外层纵肌层与其余组织层分离，采用免疫荧光技术对七种神经肽进行染色，并用徕卡Orthoplan荧光显微镜进行研究。所有研究材料对所有神经肽检测均呈阳性。得出的结论是，所有检测的肽在所有羔羊中均存在，因此致死羔羊的肌间神经节具有功能。
