Nakazono M, Tsutsumi N, Sugiura M, Hirai A
Laboratory of Radiation Genetics, Faculty of Agriculture, University of Tokyo, Japan.
Plant Mol Biol. 1995 May;28(2):343-6. doi: 10.1007/BF00020253.
The 76 bp sequence found in the upstream region of a gene for 26S rRNA (rrn26) is duplicated in the upstream region of a gene for initiator methionine tRNA (trnfM) in the mitochondrial genome of rice. An in vitro capping/ribonuclease protection assay and primer extension analysis demonstrated that the transcription of trnfM and of rrn26, which are at least 190 kb from one another in the rice mitochondrial genome, starts from these same sequences in the upstream regions of the respective genes. This result indicates that the short sequence that is duplicated in the upstream regions of trnfM and rrn26 in rice mtDNA is recognized as the promoter of each respective gene.
在水稻线粒体基因组中，26S rRNA（rrn26）基因上游区域发现的76 bp序列，在起始甲硫氨酸tRNA（trnfM）基因的上游区域发生了重复。体外加帽/核糖核酸酶保护分析和引物延伸分析表明，在水稻线粒体基因组中彼此相距至少190 kb的trnfM和rrn26的转录，起始于各自基因上游区域的这些相同序列。这一结果表明，水稻线粒体DNA中trnfM和rrn26上游区域重复的短序列被识别为各自基因的启动子。
