Graduate Division of Biochemical Regulation, School of Agriculture, Nagoya University, 464-01, Nagoya, Japan.
Theor Appl Genet. 1992 Jul;84(3-4):275-9. doi: 10.1007/BF00229482.
Mitochondrial DNA (mtDNA) was isolated from young green leaves of rice plants. DNA fragments were cloned into lambda DNA, and clones that hybridized to mitochondrial genes from other plants were selected. Distal restriction fragments of these clones were used as probes for the selection of overlapping clones. A genetic map was finally created from the library by "walking" along the genome. The mitochondrial genome consists of five basic circles, with each circle sharing homologous sequences with one or two other circles. A master circle was constructed from the results of recombination across repeated sequences, and its size was estimated to be 492 kb. A physical map and a bank of overlapping clones were also constructed.
从水稻幼嫩的绿色叶片中提取线粒体 DNA（mtDNA）。将 DNA 片段克隆到 λ 噬菌体 DNA 中，并筛选出与其他植物线粒体基因杂交的克隆。用这些克隆的远端限制酶切片段作为探针，选择重叠克隆。最后通过基因组“行走”，从文库中构建遗传图谱。线粒体基因组由五个基本环组成，每个环与一个或两个其他环共享同源序列。主环是由重复序列的重组结果构建的，其大小估计为 492kb。还构建了物理图谱和重叠克隆库。
