Ma Y, Ito Y
Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
J Chromatogr A. 1995 May 19;702(1-2):197-206. doi: 10.1016/0021-9673(94)00852-z.
pH-Zone-refining counter-current chromatography was applied to the preparative separation of oligopeptide derivatives containing up to three amino acids. Both acidic benzyloxycarbonyl peptides and basic peptides-beta-naphthylamide were successfully separated with two-phase solvent systems composed of methyl tert.-butyl ether, 1-butanol, acetonitrile and water using a set of suitable retainer and eluent reagents for each group. The preparative ability of the method was demonstrated in the separation of multigram quantities of analyte with a standard separation column with a total capacity of 325 ml. The effects of important factors such as polarity of the two-phase solvent system, concentrations of the eluent base and the retainer acid were discussed.
pH区带精制逆流色谱法被应用于含至多三个氨基酸的寡肽衍生物的制备分离。使用由甲基叔丁基醚、1-丁醇、乙腈和水组成的两相溶剂体系，分别针对酸性苄氧羰基肽和碱性肽-β-萘酰胺这两类化合物，通过使用一组合适的保留剂和洗脱剂，成功实现了它们的分离。该方法的制备能力通过使用总容量为325 ml的标准分离柱对多克量分析物进行分离得到了证明。文中还讨论了两相溶剂体系极性、洗脱剂碱浓度和保留剂酸浓度等重要因素的影响。
