Ito Y, Ma Y
Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
J Chromatogr A. 1994 Jun 24;672(1-2):101-8. doi: 10.1016/0021-9673(94)80597-0.
A new mode of pH-zone-refining counter-current chromatography operates in a manner analogous to displacement chromatography. The method uses a retainer base (acid) in the stationary phase to retain analytes in the column and a displacer acid (base) to elute the analytes in the decreasing order of pKa and hydrophobicity. The elution produces a train of highly concentrated rectangular solute peaks with minimum overlap. Utility of the method is demonstrated in the separation of dinitrophenyl amino acids in a two-phase solvent system composed of methyl tert.-butyl ether and water. Compared with the original mode of pH-zone-refining counter-current chromatography, the present mode is more amenable to ligand-affinity separation which may cover a broader range of analytes including non-ionizable compounds.
一种新型的pH区带精制逆流色谱以类似于置换色谱的方式运行。该方法在固定相中使用保留碱（酸）将分析物保留在柱中，并使用置换酸（碱）以pKa和疏水性递减的顺序洗脱分析物。洗脱产生一系列高度浓缩的矩形溶质峰，重叠最小。该方法的实用性在由甲基叔丁基醚和水组成的两相溶剂系统中分离二硝基苯基氨基酸时得到了证明。与原始的pH区带精制逆流色谱模式相比，本模式更适合配体亲和分离，这种分离可能涵盖更广泛的分析物，包括非离子化化合物。
