Swain S L
Department of Biology, University of California, San Diego, La Jolla 92093-0063, USA.
Immunity. 1994 Oct;1(7):543-52. doi: 10.1016/1074-7613(94)90044-2.
In vitro generated CD4 cell lines and effectors often produce either Th1 or Th2 cytokines, but stimulation of resting CD4 cells from animals leads to production mostly of IL-2. To determine whether polarization of CD4 effector cells results in development of polarized memory, I generated antigen-specific Th1 and Th2 effectors in vitro and transferred them to adoptive hosts. The effectors gave rise to long-lived populations of CD4 T cells with the phenotype of resting memory cells. Recovered cells responded vigorously to specific antigen, producing a pattern of cytokines closely related to that of the transferred effectors. Thus, encounter with a peptide antigen and directive cytokines at the initiation of culture can lead to generation of both effector and long-lived memory cell populations that produce restricted patterns of cytokines.
体外产生的CD4细胞系和效应细胞通常产生Th1或Th2细胞因子，但刺激动物体内静止的CD4细胞主要导致产生IL-2。为了确定CD4效应细胞的极化是否会导致极化记忆的形成，我在体外产生了抗原特异性的Th1和Th2效应细胞，并将它们转移到过继宿主中。这些效应细胞产生了具有静止记忆细胞表型的长寿CD4 T细胞群体。回收的细胞对特异性抗原产生强烈反应，产生的细胞因子模式与转移的效应细胞密切相关。因此，在培养开始时与肽抗原和定向细胞因子相遇可导致产生效应细胞和长寿记忆细胞群体，它们产生受限的细胞因子模式。
