Pazzi K A, Kraegel S A, Griffey S M, Theon A P, Madewell B R
Department of Surgical and Radiological Sciences, University of California, School of Veterinary Medicine, Davis 95616, USA.
Cancer Lett. 1996 Oct 1;107(1):125-30. doi: 10.1016/0304-3835(96)04359-5.
Wild type equine p53 was amplified between exons 2 and 9 by the polymerase chain reaction using primers designed from conserved regions in other species. An 828 base pair region, corresponding to codons 25-313 of human p53, was sequenced in both directions. Human and equine amino acid sequences were 87% homologous in this region and 96% homologous in conserved domains II-V. Of eight equine cutaneous or mucocutaneous squamous cell carcinomas directly sequenced from exons 5-8, two had p53 point mutations resulting in single amino acid substitutions.
使用从其他物种保守区域设计的引物，通过聚合酶链反应扩增野生型马p53基因的外显子2至9之间的区域。对一个对应于人p53密码子25 - 313的828个碱基对区域进行双向测序。在该区域，人和马的氨基酸序列同源性为87%，在保守结构域II - V中同源性为96%。对直接从外显子5 - 8测序的8例马皮肤或黏膜皮肤鳞状细胞癌进行分析，其中2例存在p53点突变，导致单个氨基酸替换。
