Bucher K, Szalai G, Marti E, Griot-Wenk M E, Lazary S, Pauli U
Division of Immunogenetics, University of Berne, Switzerland.
Res Vet Sci. 1996 Sep;61(2):114-9. doi: 10.1016/s0034-5288(96)90084-3.
The tumour suppressor protein p53 enhances the genetic stability of the cell and plays a critical role in tumour suppression. Equine p53 was analysed by sequencing exons 5 to 9, a region which includes most known mutations and all the mutational hotspots in the species that have been investigated. The fragment was amplified, cloned and sequenced from genomic and complementary DNA. A comparison of the predicted amino acid sequences between the horse and other species resulted in identities between 66 per cent with the clawed frog and 92 per cent with the cat. Using the single strand conformation polymorphism technique, exons 5 to 8 amplified from sarcoid tissue and peripheral leucocytes of 28 sarcoid-affected and 11 healthy horses were screened for mutations. No mutations were identified, suggesting that the frequency of p53 mutations in equine sarcoid might be low. However, the high incidence of bovine papillomavirus (BPV) infection in equine sarcoid may indicate the functional inactivation of p53 by BPV-encoded E6 protein.
肿瘤抑制蛋白p53可增强细胞的遗传稳定性，并在肿瘤抑制中发挥关键作用。通过对5至9号外显子进行测序来分析马的p53，该区域包含了该物种中大多数已知突变以及所有已研究的突变热点。从基因组DNA和互补DNA中扩增、克隆并测序该片段。对马与其他物种的预测氨基酸序列进行比较，结果显示与爪蟾的一致性为66%，与猫的一致性为92%。使用单链构象多态性技术，对28匹患有肉瘤病和11匹健康马的肉瘤组织和外周血白细胞中扩增出的5至8号外显子进行突变筛查。未发现突变，这表明马肉瘤中p53突变的频率可能较低。然而，马肉瘤中牛乳头瘤病毒（BPV）感染的高发生率可能表明BPV编码的E6蛋白使p53功能失活。
