恶性疟原虫4号染色体的酵母人工染色体连续克隆系图谱：两个近期分离株之间DNA扩增的特征分析

A YAC contig map of Plasmodium falciparum chromosome 4: characterization of a DNA amplification between two recently separated isolates.

作者信息

Rubio J P, Triglia T, Kemp D J, de Bruin D, Ravetch J V, Cowman A F

机构信息

Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Victoria, Australia.

出版信息

Genomics. 1995 Mar 20;26(2):192-8. doi: 10.1016/0888-7543(95)80200-6.

DOI:10.1016/0888-7543(95)80200-6

PMID:7601442

Abstract

We have generated a physical map of Plasmodium falciparum chromosome 4 using yeast artificial chromosomes (YACs). The map is defined by a YAC contig spanning approximately 1.05 Mb, which has been restriction mapped to a resolution of 30 kb and is punctuated by 22 sequence-tagged sites. The physical information obtained has enabled us to compare and contrast the structure of chromosome 4 in detail between FCR3 and B8, two recently separated isolates of P. falciparum, leading to characterization of a novel chromosome polymorphism occurring in a subtelomeric region. Comparison of chromosomes 4 from 10 different isolates has shown that chromosome size polymorphisms are restricted to both subtelomeric regions. These analyses provide a high-resolution physical map that will be important to complement genetic analysis of this human pathogen.

摘要

我们利用酵母人工染色体（YAC）构建了恶性疟原虫4号染色体的物理图谱。该图谱由一个跨越约1.05 Mb的YAC重叠群定义，该重叠群已被限制酶切图谱分析至30 kb的分辨率，并由22个序列标签位点标记。所获得的物理信息使我们能够详细比较和对比FCR3和B8这两个最近分离的恶性疟原虫分离株中4号染色体的结构，从而鉴定出一个发生在亚端粒区域的新型染色体多态性。对来自10个不同分离株的4号染色体进行比较表明，染色体大小多态性仅限于两个亚端粒区域。这些分析提供了一个高分辨率的物理图谱，这对于补充对这种人类病原体的遗传分析将具有重要意义。