Werner E, Holder A A, Hoheisel J D
Molecular-Genetic Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 506, D-69120 Heidelberg, Germany.
Nucleic Acids Res. 1997 Apr 1;25(7):1467-8. doi: 10.1093/nar/25.7.1467.
To date, frozen storage of YAC libraries have relied on the administration of glycerol to the medium subsequent to cell growth. By adding Hogness Freezing Medium prior to inoculation, cultures can be frozen directly after cell growth, with no adverse effect on the stability of the YAC DNA or on the viability of the cells even after repeated freezing and defrosting. Although a relatively simple modification, the procedure notably improves the handling of YAC libraries and significantly reduces the risk of contamination, especially when dealing with large numbers of clones.
迄今为止，酵母人工染色体（YAC）文库的冷冻保存一直依赖于在细胞生长后向培养基中添加甘油。通过在接种前添加霍格尼斯冷冻培养基，培养物在细胞生长后即可直接冷冻，即使经过反复冻融，对YAC DNA的稳定性或细胞活力也没有不利影响。尽管这是一个相对简单的改进，但该程序显著改善了YAC文库的处理方式，并显著降低了污染风险，尤其是在处理大量克隆时。
