Muntané J, Longo V, Mitjavila M T, Gervasi P G, Ingelman-Sundberg M
Laboratory of Genetics and Biochemical Toxicology, Instituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy.
Inflammation. 1995 Apr;19(2):143-56. doi: 10.1007/BF01534458.
Carrageenan-induced granuloma was used to study the apoprotein and RNA content, and catalytic activities of several cytochrome P-450 isozymes in liver. This model allowed discrimination between acute and chronic phases of experimental inflammation. The expression of most isozymes studied (CYP2D, CYP2E1, CYP3A1 and CYP4A) was reduced to 20% of the control level during the acute phase and partially recovered (30-60% of control group) during the chronic phase. CYP2B1 content was decreased to 65% of control during the acute and chronic phases of inflammation. RNA (CYP2B1 and CYP2E1) showed a strong depression during the acute phase and recovered during the chronic phase, without differences between isoenzymes. In most cases, there was a good correlation between the apoprotein content of isozymes and related activities. Our results show that the depletion of cytochrome P-450 induced by inflammation depends on the severity of the disease. Experimental inflammation equally affect the transcription of CYP2B1 and CYP2E1, so differences in apoprotein content and related activities between isozymes may due to differential posttranscriptional regulation.
角叉菜胶诱导的肉芽肿被用于研究肝脏中几种细胞色素P-450同工酶的载脂蛋白和RNA含量以及催化活性。该模型能够区分实验性炎症的急性期和慢性期。在急性期，大多数所研究的同工酶（CYP2D、CYP2E1、CYP3A1和CYP4A）的表达降至对照水平的20%，而在慢性期部分恢复（对照组的30-60%）。在炎症的急性期和慢性期，CYP2B1含量降至对照的65%。RNA（CYP2B1和CYP2E1）在急性期表现出强烈抑制，在慢性期恢复，同工酶之间无差异。在大多数情况下，同工酶的载脂蛋白含量与相关活性之间存在良好的相关性。我们的结果表明，炎症诱导的细胞色素P-450的消耗取决于疾病的严重程度。实验性炎症同样影响CYP2B1和CYP2E1的转录，因此同工酶之间载脂蛋白含量和相关活性的差异可能归因于转录后调控的差异。
