一种来自乳酸乳球菌双乙酰亚种的新型质粒编码噬菌体流产感染系统。
A novel plasmid-encoded phage abortive infection system from Lactococcus lactis biovar. diacetylactis.
作者信息
Deng Y M, Harvey M L, Liu C Q, Dunn N W
机构信息
Department of Biotechnology, University of New South Wales, Sydney, Australia.
出版信息
FEMS Microbiol Lett. 1997 Jan 1;146(1):149-54. doi: 10.1111/j.1574-6968.1997.tb10185.x.
A 16-kb plasmid (pND859) was identified from Lactococcus lactis biovar. diacetylactis UK12922 which encodes phage resistance to the small isometric phage 712 when tested in L. lactis LM0230. The gene encoding phage abortive infection, designated abi-859, was localized on a 1.2-kb region which consists of an open reading frame (ORF) of 846 bp preceded by a potential ribosome-binding site and a putative promoter region. A helix-turn-helix region typical of DNA-binding motifs was identified near the N-terminal of the abi-859 product, suggesting a possible interaction with the phage DNA.
从乳酸乳球菌双乙酰亚种UK12922中鉴定出一个16 kb的质粒（pND859），当在乳酸乳球菌LM0230中进行测试时，该质粒编码对小型等轴噬菌体712的噬菌体抗性。编码噬菌体流产感染的基因，命名为abi-859，定位在一个1.2 kb的区域，该区域由一个846 bp的开放阅读框（ORF）组成，前面有一个潜在的核糖体结合位点和一个推定的启动子区域。在abi-859产物的N端附近鉴定出一个典型的DNA结合基序的螺旋-转角-螺旋区域，表明其可能与噬菌体DNA相互作用。
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