Müller H P, Eickholz P, Heinecke A, Pohl S, Müller R F, Lange D E
Department of Operative Dentistry and Periodontology, University of Heidelberg, Germany.
J Clin Periodontol. 1995 May;22(5):413-9. doi: 10.1111/j.1600-051x.1995.tb00169.x.
In the present study, a total of 619 subgingival and extracrevicular samples from 66 early-onset periodontitis, 42 adult periodontitis/gingivitis and 36 treated Actinobacillus actinomycetemcomitans-associated periodontitis patients were selectively cultivated for presence of A. actinomycetemcomitans. The organism was recovered from 68% cases with early-onset periodontitis, 24% cases with adult periodontitis/gingivitis and 50% of treated patients. Associations between recovery from pooled subgingival plaque and samples from extracrevicular locations as well as between different extracrevicular samples, were not heterogeneous with regard to different groups with the exception for cheek/saliva comparisons (odds ratios: early-onset periodontitis 825; adult periodontitis 8.1; treated patients 117; 0.05 < p < 0.1). For associations between recovery of A. actinomycetemcomitans from pooled subgingival plaque/extracrevicular samples, Mantel-Haenszel's odds ratios of between 12.2 and 21.6 were calculated (p < 0.0001). The organism was isolated from 17 cheek mucosa samples of 18 patients identified as still harboring the organism after therapy. Present results point to the considerable value of cheek mucosa samples especially in treated patients to diagnose persistent A. actinomycetemcomitans colonization of the oral cavity.
在本研究中，从66例早发性牙周炎、42例成人牙周炎/牙龈炎以及36例经治疗的伴放线放线杆菌相关牙周炎患者中，共选择性采集了619份龈下和龈外样本，以检测伴放线放线杆菌的存在情况。在早发性牙周炎患者中，该菌的检出率为68%；在成人牙周炎/牙龈炎患者中为24%；在经治疗的患者中为50%。除了脸颊/唾液比较外（优势比：早发性牙周炎825；成人牙周炎8.1；经治疗患者117；0.05 < p < 0.1），在不同组中，从龈下菌斑混合样本与龈外部位样本中检出该菌的相关性，以及不同龈外样本之间的相关性并无差异。对于从龈下菌斑混合样本/龈外样本中检出伴放线放线杆菌的相关性，计算出Mantel-Haenszel优势比在12.2至21.6之间（p < 0.0001）。从18例经治疗后仍被鉴定为携带该菌的患者的17份脸颊黏膜样本中分离出了该菌。目前的结果表明，脸颊黏膜样本具有相当大的价值，尤其是对于经治疗的患者，有助于诊断口腔中伴放线放线杆菌的持续定植情况。
