Roy R, Reynolds R
N.S.P. Criminalistics Laboratory, Lincoln, NE, USA.
J Forensic Sci. 1995 Mar;40(2):266-9.
Deoxyribonucleic acid (DNA) samples extracted from stained pap smear, semen smear and postcoital slides were amplified by the polymerase chain reaction (PCR) and typed for the AmpliType PM and HLA DQ alpha alleles. HLA DQ alpha and PM types consistent with blood controls from the donors were obtained. Stained cells fixed to slides can provide a valuable source of material for determining a genetic profile of the sample donor, particularly in sexual assault cases and possibly in missing person cases involving women.
从染色的巴氏涂片、精液涂片和性交后玻片上提取的脱氧核糖核酸(DNA)样本,通过聚合酶链反应(PCR)进行扩增,并对AmpliType PM和人类白细胞抗原DQα等位基因进行分型。获得了与供体血液对照一致的人类白细胞抗原DQα和PM分型。固定在玻片上的染色细胞可为确定样本供体的基因图谱提供有价值的材料来源,特别是在性侵犯案件中,可能在涉及女性的失踪人员案件中也是如此。