Fildes N, Reynolds R
Department of Human Genetics, Roche Molecular Systems, Inc., Alameda, CA, USA.
J Forensic Sci. 1995 Mar;40(2):279-86.
The AmpliType PM Field Trial was designed to assess the ability of forensic laboratories to obtain the correct results from samples commonly encountered in forensic casework. The seven forensic laboratory participants of the AmpliType PM Field Trial each performed four studies. Samples were analyzed using components of the AmpliType PM PCR Amplification and Typing Kit. Laboratories were also provided with DNA probe strips to type the DQA1 locus. Of the 381 PM and 325 DQA1 DNA probe strip results obtained from DNA-containing and non-DNA-containing samples, 98.2% and 95.7% showed the correct result for PM and DQA1 types, respectively. No samples were typed incorrectly. The remaining small percentage of samples were either uninterpretable due to the presence of a mixture, or no result was obtained due to insufficient DNA. The Field Trial demonstrated that laboratories can easily implement the AmpliType PM system to analyze DNA-containing samples and controls successfully for forensic casework applications.
AmpliType PM现场试验旨在评估法医实验室从法医案件工作中常见样本获得正确结果的能力。AmpliType PM现场试验的七家法医实验室参与者各自进行了四项研究。使用AmpliType PM PCR扩增和分型试剂盒的组件对样本进行分析。还向实验室提供了用于对DQA1基因座进行分型的DNA探针条。从含DNA和不含DNA的样本中获得的381个PM和325个DQA1 DNA探针条结果中，分别有98.2%和95.7%的结果显示PM和DQA1类型正确。没有样本分型错误。其余小部分样本要么由于存在混合物而无法解读，要么由于DNA不足而未获得结果。现场试验表明，实验室可以轻松实施AmpliType PM系统，以成功分析含DNA的样本和对照，用于法医案件工作应用。
