Cacciapuoti B, Ciceroni L, Ciarrocchi S, Khoury C, Simeoni J
Istituto Superiore di Sanità, Department of Bacteriology and Medical Mycology, Rome, Italy.
New Microbiol. 1995 Apr;18(2):169-81.
Lyme disease is caused by three borrelial species, B. burgdorferi sensu stricto, B. garinii and Borrelia group VS461. In a restricted biotope of the Bolzano province, in the Caldaro community, five clones of two borrelial variants were isolated from Ixodes ricinus ticks. A preliminary serological study showed that the two variants cross-reacted with B. burgdorferi B31 and B. garinii N34 strains, respectively. The isolates were genomically related with strains B31 and N34, respectively, sharing a similar plasmid and restriction fragment length polymorphism profile with these strains. The phenotypic pattern of the Caldaro isolates-namely their protein and antigenic profile-showed infra-subspecific variation compared to related strains B31 and N34 respectively. The observed phenotypic variability between strains isolated from the same biotope and in the same tick host strongly indicated the variability of gene-encoded characters is a constant characteristic of borrelial strains, even when from the same ecological niche.
莱姆病由三种疏螺旋体引起，即狭义伯氏疏螺旋体、伽氏疏螺旋体和VS461疏螺旋体群。在博尔扎诺省卡尔达罗社区的一个受限生物群落中，从蓖麻硬蜱中分离出了两个疏螺旋体变种的五个克隆。一项初步血清学研究表明，这两个变种分别与伯氏疏螺旋体B31菌株和伽氏疏螺旋体N34菌株发生交叉反应。这些分离株在基因组上分别与B31菌株和N34菌株相关，与这些菌株具有相似的质粒和限制性片段长度多态性图谱。与相关菌株B31和N34相比，卡尔达罗分离株的表型模式，即其蛋白质和抗原图谱，显示出亚亚种以下的变异。在同一生物群落和同一蜱宿主中分离出的菌株之间观察到的表型变异性强烈表明，即使来自相同的生态位，基因编码特征的变异性也是疏螺旋体菌株的一个恒定特征。
